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Fluorescent Detection of 2,4-Dichlorophenoxyacetic Acid in Food Samples Based on Covalent Organic Frameworks and MnO2 Nanosheets

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Abstract

2,4-Dichlorophenoxyacetic acid (2,4-D) is a typical chlorinated aromatic herbicide which is widely used because of its low cost. Herein, a new fluorescent assay for 2,4-D is developed based on covalent organic frameworks (COFs) and MnO2 nanosheets. The fluorescence of COFs is quenched effectively by MnO2 nanosheets. Ascorbic acid 2-phosphate (AAP) is hydrolyzed by alkaline phosphatase (ALP) to produce ascorbic acid (AA), which reduces MnO2 nanosheets into Mn2+, so the quenched fluorescence recovers. Additionally, 2,4-D can inhibit the catalytic activity of ALP. Therefore, the addition of 2,4-D quenches the fluorescence of COF-MnO2 system again. Under the optimum conditions, this assay displays a wide linear range of 2,4-D from 1 to 150,000 ng/mL with a limit of detection of 0.36 ng/mL. Then, this method is successfully used to measure 2,4-D in rice, millet, and cucumber samples with satisfactory recoveries. Compared with other probes for 2,4-D detection, both of COFs and MnO2 nanosheets are stable, cost-effective, and easily prepared. The sensitivity and linear range of 2,4-D in this strategy are superior to most reported works. This is the first time that a novel sensing platform using fluorescent COFs and MnO2 nanosheets is developed for 2,4-D.

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Funding

This work was supported by Natural Science Foundation of Shandong Province, China (ZR2019QB010), and the Scientific Research Foundation of Qufu Normal University (BSQD20130117).

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Authors and Affiliations

  1. The Key Laboratory of Life-Organic Analysis, Qufu Normal University, Qufu, 273165, Shandong, China

    Fei Qu, Yang Sun, Shuxian Guo, Hang Yan & Jinmao You

  2. The Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine, Qufu Normal University, Qufu, 273165, Shandong, China

    Fei Qu, Yang Sun, Shuxian Guo, Hang Yan & Jinmao You

  3. The Key Laboratory of Tibetan Medicine Research & Qinghai Provincial Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Science, Xining, 810001, China

    Jinmao You

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  1. Fei Qu
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Contributions

Fei Qu and Yang Sun contributed equally to this work.

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Correspondence to Fei Qu.

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This study does not contain experiments that involve human or animals other than the authors who performed the work.

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Fei Qu declares that she has no conflict of interest. Yang Sun declares that she has no conflict of interest. Shuxian Guo declares that she has no conflict of interest. Hang Yan declares that she has no conflict of interest. Jinmao You declares that he has no conflict of interest.

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Qu, F., Sun, Y., Guo, S. et al. Fluorescent Detection of 2,4-Dichlorophenoxyacetic Acid in Food Samples Based on Covalent Organic Frameworks and MnO2 Nanosheets. Food Anal. Methods 13, 1842–1851 (2020). https://doi.org/10.1007/s12161-020-01807-2

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