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Development and validation of a novel analytical method to quantify aflatoxins in baby food samples by employing dispersive solid phase extraction with multi-walled carbon nanotubes

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Abstract

A new method for the quantification of four aflatoxins (AFB1, AFB2, AFG1, and AFG2) in baby food samples is described herein. In this method, the extraction/cleanup step was performed by using multi-walled carbon nanotubes as a sorbent material in a dispersive solid phase procedure. In sequence, these mycotoxins were quantified via liquid chromatographic coupled with tandem mass spectrometry. The method was fully validated according to the EC/657/2002 and SANCO/12571/2013 directives. Adequate values were obtained for all figures of merit, mainly veracity (recoveries: 75.8 to 120.6%) and quantification limits (LOQs: 60.0 to 560.0 ng kg-1), with no noticeable matrix effects. The method was tested in real samples (n = 4) and aflatoxin B1 (AFB1) was detected in just one of them. Finally, this analytical procedure has an evident potential to be applied to analyze aflatoxins (and other mycotoxins) in distinct types of complex samples.

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Authors and Affiliations

  1. Departamento de Química, Instituto de Ciências Exatas, Universidade Federal de Minas Gerais, Avenida Presidente Antônio Carlos, 6627, Pampulha, Belo Horizonte, MG, 31270-901, Brazil

    Lucas Pinto da Silva, Adriana Ferreira Faria & Rodinei Augusti

  2. Laboratório de Controle de Qualidade e Segurança Alimentar (LACQSA), Avenida Raja Gabaglia, 245, Setor H, Bairro Cidade Jardim, Belo Horizonte, MG, 30380-103, Brazil

    Eugenia Azevedo Vargas & Fernando Diniz Madureira

Authors
  1. Lucas Pinto da Silva
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  2. Eugenia Azevedo Vargas
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  3. Fernando Diniz Madureira
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  4. Adriana Ferreira Faria
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  5. Rodinei Augusti
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Correspondence to Rodinei Augusti.

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Conflict of Interest

Lucas Pinto da Silva declares that he has no conflict of interest. Eugênia de Azevedo Vargas declares that she has no conflict of interest. Fernando Diniz Madureira declares that he has no conflict of interest. Adriana Ferreira Faria declares that she has no conflict of interest. Rodinei Augusti declares that he has no conflict of interest. Josep V. Mercader declares that he has no conflict of interest.

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All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted.

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da Silva, L.P., Azevedo Vargas, E., Madureira, F.D. et al. Development and validation of a novel analytical method to quantify aflatoxins in baby food samples by employing dispersive solid phase extraction with multi-walled carbon nanotubes. Food Anal. Methods 13, 1530–1537 (2020). https://doi.org/10.1007/s12161-020-01778-4

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  • DOI: https://doi.org/10.1007/s12161-020-01778-4

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