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Development and Validation of an Analytical Method for the Determination of Aloin Using Liquid Chromatography Tandem Mass Spectrometry

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Abstract

A new simple analytical method was developed for the identification and quantification of aloin in aloe gel. The extraction was performed using acetonitrile acidified with formic acid (1%, v/v) with no additional cleanup step. The compound was determined using high-performance liquid chromatography coupled with triple quadrupole mass spectrometry (QqQ). The recovery rates obtained were between 80 and 110% with a relative standard deviation < 26%, at four different concentration levels. The calculated limit of quantification (LOQ) was at 0.05 mg kg−1.

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Authors and Affiliations

  1. Department of Pesticides Control and Phytopharmacy, Laboratory of Pesticides’ Residues, Benaki Phytopathological Institute, 8 St. Delta Street, Kifissia, 14561, Athens, Greece

    Chris Anagnostopoulos & George Ampadogiannis

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  1. Chris Anagnostopoulos
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  2. George Ampadogiannis
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Correspondence to Chris Anagnostopoulos.

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Chris Anagnostopoulos declares that he has no conflict of interest. George Ampadogiannis declares that he has no conflict of interest.

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Anagnostopoulos, C., Ampadogiannis, G. Development and Validation of an Analytical Method for the Determination of Aloin Using Liquid Chromatography Tandem Mass Spectrometry. Food Anal. Methods 13, 1409–1420 (2020). https://doi.org/10.1007/s12161-020-01756-w

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