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Tracing Atlantic Salmon (Salmo salar) in Processed Fish Products Using the Novel Loop-Mediated Isothermal Amplification (LAMP) and PCR Assays

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Abstract

Due to the legislative shortcomings, Atlantic salmon often shares the common name of San Wen Yu (Chinese ideogram 三文鱼) with other salmonids in China, therefore concealing the differences with the rest salmonids in price, nutrition, and even food safety aspect, and also paving the way for species adulteration. To help prevent this episode, the present study aimed to develop novel loop-mediated isothermal amplification (LAMP) and PCR assays for rapid identification of Atlantic salmon in processed fish products. Primers (LAMP and PCR) specific for Atlantic salmon were designed and the specificity was successfully confirmed. The sensitivity of the PCR assay was about 10-fold higher than the LAMP assay. Direct detection of the LAMP-positive samples using SYBR green in daylight or under UV light was also validated, making the total analysis time of 80 min, far less than the PCR assay. Finally, the developed methods were successfully applied to commercially San Wen Yu products, and the presence of Atlantic salmon was highlighted in only six products.

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Funding

This work was supported by the National Key Research and Development Program of China (No. 2018YFC1602800) and the National Natural Science Foundation of China (grant number 31701688).

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Author notes

  1. Xiong Xiong and Manhong Huang contributed equally to this work.

Authors and Affiliations

  1. College of Food Science and Light Industry, Nanjing Tech University, No. 30 Puzhu Road South, Nanjing, 211800, China

    Xiong Xiong, Manhong Huang, Wenjie Xu, Min Cao, Yi Li & Xiaohui Xiong

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  1. Xiong Xiong
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  2. Manhong Huang
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  4. Min Cao
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Correspondence to Xiong Xiong.

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Xiong Xiong declares that he has no conflict of interest. Manhong Huang declares that she has no conflict of interest. Wenjie Xu declares that he has no conflict of interest. Min Cao declares that she has no conflict of interest. Yi Li declares that he has no conflict of interest. Xiaohui Xiong declares that he has no conflict of interest.

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Xiong, X., Huang, M., Xu, W. et al. Tracing Atlantic Salmon (Salmo salar) in Processed Fish Products Using the Novel Loop-Mediated Isothermal Amplification (LAMP) and PCR Assays. Food Anal. Methods 13, 1235–1245 (2020). https://doi.org/10.1007/s12161-020-01738-y

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