Abstract
Avermectin (AVM) has been widely used in agriculture and animal husbandry for the treatment of broad spectrum parasitic diseases, but it has a harmful effect on human body through food chain. In this paper, on the basis of anti-AVM specific antibody, expanded mesoporous silica-encapsulated gold nanoparticles (EMSN-AuNPs)-based enzyme-linked immunosorbent assay (ELISA) for the detection of AVM was developed. Under the optimized conditions, the linear range of the developed EMSN-AuNPs-based ELISA was from 2.53 to 184.28 ng/mL, with the limit of detection (LOD) of 2.17 ng/mL. In addition, the cross-reactivity to macrolide antibiotic analogues was less than 0.1%. Therefore, the established EMSN-AuNPs-based ELISA was used to detect the AVM residues in the spiked samples, with the recovery from 99.5 to 101.5%, and the results was further validated by high-performance liquid chromatography (HPLC) and ELISA. Therefore, the constructed EMSN-AuNPs-based ELISA is suitable for monitoring AVM in biological samples with its sensitivity, accuracy, and real-timing.
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This work was financially supported by the Guangzhou Science and Technology Foundation (2016201604030025, 201902010430100011 and 201903010034), Guangdong Science and Technology Foundation (2017A050501034).
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This article does not contain any studies with human subjects. All animal experiments that described in the present study were performed in adherence to the Guangdong Animal Center guidelines and approved by Animal Ethics Committee.
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Zhang, C., Zhong, Y., He, Q. et al. Positively Charged Nanogold Combined with Expanded Mesoporous Silica-Based Immunoassay for the Detection of Avermectin. Food Anal. Methods 13, 1129–1137 (2020). https://doi.org/10.1007/s12161-020-01732-4
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DOI: https://doi.org/10.1007/s12161-020-01732-4