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Determination of the Water Activities of Wines and Spirits

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Abstract

Water activity (aw) is an important property of foods, with correlations to safety, quality, and shelf-life. The presence of high concentrations of non-water volatiles has been problematic for analytical aw instruments, thereby limiting potential applications of aw measurements for quality assessments of fermented beverages and foods. The objectives of this study were to measure the aw values (aws) of wines and spirits using a tunable diode laser instrument (AquaLab TDL, METER Group, Inc.), reported to be unaffected by volatiles such as ethanol, and determine the effects of ethanol and residual sugar (R.S.) concentrations on the aw. The aws of commercial wines (n = 678), other liquors (n = 42), and model solutions containing controlled concentrations of ethanol and sugars were measured using the TDL at 25 °C. The alcohol by volume (ABV) was determined by electric ebulliometer and Fourier transform infrared spectroscopy (FTIR) methods, and sugars were determined using a FTIR method. The aws of wines ranged from 0.860 to 0.968 (average 0.940 aw), the aws of spirits ranged from 0.750 to 0.909 aw, and grain alcohol had the lowest aw at 0.365 and the highest ABV (95%). The Norrish equation, accounting for ethanol, glucose, fructose, and sucrose concentrations, resulted in predicted aws of wines that were 0.012 ± 0.007 higher than the measured aws. Ethanol had a greater effect on the aw of wines than sugar contents, and the ~ 0.012 lower than predicted aws of wines were attributed to the effects of additional solutes (glycerol, acids), that were not included in the Norrish equation, on lowering the aw.

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References

  • Alcohol and Tobacco Tax and Trade Bureau (2018) Tax and fee rates. https://www.ttb.gov/tax_audit/taxrates.shtml#Wine18

  • Allan M, Mauer LJ (2017) Dataset of water activity measurements of alcohol: water solutions using a tunable diode laser. Data Brief 12:364–369

    Article  Google Scholar 

  • Bamforth CW (2008) Food, fermentation and micro-organisms. John Wiley & Sons

  • Bauer R, Nieuwoudt H, Bauer FF, Kossmann J, Koch KR, Esbensen KH (2008) FTIR spectroscopy for grape and wine analysis. Anal Chem 80:1371–1379

    Article  CAS  Google Scholar 

  • Boulton RB, Singleton VL, Bisson LF, Kunkee RE (1999) Yeast and biochemistry of ethanol fermentation. In: Principles and practices of winemaking. Springer US, Boston, pp 102–192. https://doi.org/10.1007/978-1-4757-6255-6_4

    Chapter  Google Scholar 

  • Brick J (2006) Standardization of alcohol calculations in research. Alcohol Clin Exp Res 30:1276–1287

    Article  CAS  Google Scholar 

  • Butzke C (2012) Wine appreciation, 2nd edn. Kendall Hunt Publishing Company, Dubuque

    Google Scholar 

  • Butzke CE (2002) 2000/2001 survey of winery laboratory proficiency. Am J Enol Vitic 53:163–169

    Google Scholar 

  • Butzke CE, Ebeler SE (1999) Survey of analytical methods and winery laboratory proficiency. Am J Enol Vitic 50:461–465

    Google Scholar 

  • Campbell GS, Lewis DP (1998) Water activity and dew point temperature measuring apparatus and method. United States Patent 5,816,704

  • CFR (2018a) Alcoholic content. Code of Federal Regulations

  • CFR (2018b) Production of wine. Code of Federal Regulations

  • CFR (2018c) The standards of identity. Code of Federal Regulations

  • Chirife J, Favetto G, Fontán CF (1982) Water activity of fructose solutions in the intermediate moisture range. Lebensm Wiss Technol 15:150–160

  • Chirife J, Fontán CF, Benmergui E (1980) The prediction of water activity in aqueous solutions in connection with intermediate moisture foods IV. Aw prediction in aqueous non electrolyte solutions. Int J Food Sci Technol 15:59–70

    Article  CAS  Google Scholar 

  • Devore JL (2011) Probability and statistics for engineering and the sciences. 8th edn. Cengage Learning, Manson

    Google Scholar 

  • Fernández-Novales J, López M-I, Sánchez M-T, Morales J, González-Caballero V (2009) Shortwave-near infrared spectroscopy for determination of reducing sugar content during grape ripening, winemaking, and aging of white and red wines. Food Res Int 42:285–291. https://doi.org/10.1016/j.foodres.2008.11.008

    Article  CAS  Google Scholar 

  • Flood AE, Johns MR, White ET (1996) Mutarotation of d-fructose in aqueous-ethanolic solutions and its influence on crystallisation. Carbohydr Res 288:45–56. https://doi.org/10.1016/S0008-6215(96)90775-2

    Article  CAS  Google Scholar 

  • Fontana AJ (1998) Water activity: why it is important for food safety. In: International conference on food safety. pp 177–185

  • Horn P (1990) Apparatus and method for the measuring of dew points. Switzerland Patent 4,898,475

  • Howe PA, Ebeler SE, Sacks GL (2015) Review of thirteen years of CTS winery laboratory collaborative data. Am J Enol Vitic 66:321–339

    Article  CAS  Google Scholar 

  • Indy International Wine Competition (2019) Wine class listing. http://www.indyinternational.org/competition/classes/. Accessed 7.3.2019

  • International Organisation of Vine and Wine (2018) OIV statistical report on world Vitiviniculture. http://www.oiv.int/public/medias/6371/oiv-statistical-report-on-world-vitiviniculture-2018.pdf

  • Jackson RS (2008a) 6 - chemical constituents of grapes and wine. In: Wine Science (Third Edition). Academic Press, San Diego, pp 270–331. https://doi.org/10.1016/B978-012373646-8.50009-3

    Chapter  Google Scholar 

  • Jackson RS (2008b) 7 - fermentation. In: Wine Science (Third Edition). Academic Press, San Diego, pp 332–417. https://doi.org/10.1016/B978-012373646-8.50010-X

    Chapter  Google Scholar 

  • Jackson RS (2008c) 10 - wine Laws, authentication, and geography. In: Wine Science (Third Edition). Academic Press, San Diego, pp 577–640. https://doi.org/10.1016/B978-012373646-8.50013-5

    Chapter  Google Scholar 

  • Jeffery DW, Wilkinson KL (2014) Wine. In: Bamforth CW, Ward RE (eds) The Oxford handbook of food fermentations. Oxford Handbooks, New York, pp 54–147

    Google Scholar 

  • Jones RP, Greenfield PF (1986) Role of water activity in ethanol fermentations. Biotechnol Bioeng 28:29–40

    Article  CAS  Google Scholar 

  • Laboratoires Dujarden-Salleron (2008) Electric Ebulliometer with electronic probe brochure

  • Labuza TP, Altunakar B (2007) Water activity prediction and moisture sorption isotherms. In: Barbosa-CÃ GV, Anthony J. Fontana Jr, Shelly J. Schmidt, and Theodore P. Labuza (ed) Water Activity in Foods-Fundamentals and Applications Blackwell Publishing and the Institute of Food Technologists, pp 109–154. doi:https://doi.org/10.1002/9780470376454.ch5

  • Lee I, Park K, Lee J (2013) Precision density and volume contraction measurements of ethanol–water binary mixtures using suspended microchannel resonators. Sensors Actuators A Phys 194:62–66. https://doi.org/10.1016/j.sna.2013.01.046

    Article  CAS  Google Scholar 

  • Liu HF, Wu BH, Fan PG, Li SH, Li LS (2006) Sugar and acid concentrations in 98 grape cultivars analyzed by principal component analysis. J Sci Food Agric 86:1526–1536

    Article  CAS  Google Scholar 

  • Max J-J, Chapados C (2007) Glucose and fructose hydrates in aqueous solution by IR spectroscopy. J Phys Chem A 111:2679–2689

    Article  CAS  Google Scholar 

  • McGovern P et al (2017) Early Neolithic wine of Georgia in the South Caucasus. Proc Natl Acad Sci 114:E10309–E10318

    Article  CAS  Google Scholar 

  • METER Group (2013) Aqualab 4TE-Operator's manual. Pullman, WA

  • METER Group (2015) TDL tunable diode laser water activity Meter-Operator's manual. Pullman, WA

  • Miyawaki O, Saito A, Matsuo T, Nakamura K (1997) Activity and activity coefficient of water in aqueous solutions and their relationships with solution structure parameters. Biosci Biotechnol Biochem 61:466–469

    Article  CAS  Google Scholar 

  • Moreira JL, Santos L (2004) Spectroscopic interferences in Fourier transform infrared wine analysis. Anal Chim Acta 513:263–268

    Article  CAS  Google Scholar 

  • Norrish R (1966) An equation for the activity coefficients and equilibrium relative humidities of water in confectionery syrups. Int J Food Sci Technol 1:25–39

    Article  CAS  Google Scholar 

  • Novasina AG (2007) LabMaster-aw operating instructions. Lachen

  • Patz CD, Blieke A, Ristow R, Dietrich H (2004) Application of FT-MIR spectrometry in wine analysis. Anal Chim Acta 513:81–89. https://doi.org/10.1016/j.aca.2004.02.051

    Article  CAS  Google Scholar 

  • Raoult F-M (1887) Loi générale des tensions de vapeur des dissolvants. C R Hebd Seances Acad Sci 104:1430–1433

  • Reid DS (2007) Water activity: fundamentals and relationships. In: Barbosa-Canovas GV, Fontana AJ, Schmidt SJ, Labuza TP (eds) Water activity in foods: fundamentals and applications. First edn. Blackwell Publishing and the Institute of Food Technologists, pp 15–28

  • Rotronic AG (2009) AwTherm manual vol version 1.1. Bassersdorf

  • Rüegg M, Blanc B (1981) The water activity of honey and related sugar solutions. Lebensm Wiss Technol 14:1–6

    Google Scholar 

  • Schmidt SJ (2004) Water and solids mobility in foods. Adv Food Nutr Res 48:1–101

    Article  CAS  Google Scholar 

  • Schmidt SJ, Fontana Jr A (2007) Water Activity Values of Select Food Ingredients and Products. In: Barbosa-CÃ GV, Anthony J. Fontana Jr, Shelly J. Schmidt, and Theodore P. Labuza (ed) Water Activity in Foods-Fundamentals and Applications Blackwell Publishing and the Institute of Food Technologists, pp 413–414

  • SDBSWeb (2018) Ethyl Alcohol. National Institute of Advanced Industrial Science and Technology. https://sdbs.db.aist.go.jp

  • Suntola TS (1979) Capacitive humidity transducer. Finland patent 4,164,868,

  • The Australian Wine Research Institute (2018) Measurement of residual sugar in wine. https://www.awri.com.au/industry_support/winemaking_resources/laboratory_methods/chemical/rs/#titration

  • Weast RC (1988) Handbook of chemistry and physics. 1st student edn. CRC Press, Boca Raton

    Google Scholar 

  • Wilker KL (1992) Hydrolysis of sucrose in eastern US table wines. Am J Enol Vitic 43:381–383

    CAS  Google Scholar 

  • Yano T, Aimi T, Nakano Y, Tamai M (1997) Prediction of the concentrations of ethanol and acetic acid in the culture broth of a rice vinegar fermentation using near-infrared spectroscopy. J Ferment Bioeng 84:461–465

    Article  CAS  Google Scholar 

  • Zhu H, Yuen C, Grant DJ (1996) Influence of water activity in organic solvent + water mixtures on the nature of the crystallizing drug phase. 1. Theophylline. Int J Pharm 135:151–160

    Article  CAS  Google Scholar 

  • Zoecklein BW, Fugelsang KC, Gump BH, Nury FS (1995) Labratory procedures. In: Wine analysis and production. Springer, pp 310–516

Download references

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Authors and Affiliations

  1. Department of Food Science, Purdue University, 745 Agriculture Mall Drive, West Lafayette, IN, 47907, USA

    Matthew C. Allan, Erica N. Grush, Christian E. Butzke & Lisa J. Mauer

  2. Bindley Bioscience Center, 1203 West State Street, West Lafayette, IN, 47907, USA

    Bartek P. Rajwa

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  1. Matthew C. Allan
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  2. Erica N. Grush
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  4. Christian E. Butzke
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Correspondence to Lisa J. Mauer.

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Matthew Allan declares that he has no conflict of interest. Erica Grush declares that she has no conflict of interest. Bartek Rajwa declares that he has no conflict of interest. Christian Butzke declares that he has no conflict of interest. Lisa Mauer declares that she has no conflict of interest.

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Allan, M.C., Grush, E.N., Rajwa, B.P. et al. Determination of the Water Activities of Wines and Spirits. Food Anal. Methods 12, 2753–2763 (2019). https://doi.org/10.1007/s12161-019-01632-2

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