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Development and Application of an Optical Biosensor Immunoassay for Aflatoxin M1 in Bovine Milk

  • Harvey E. IndykEmail author
  • Sowmya Chetikam
  • Brendon D. Gill
  • Jackie E. Wood
  • David C. Woollard
Article
  • 34 Downloads

Abstract

An automated optical biosensor-based immunoassay exploiting surface plasmon resonance detection for the quantitation of aflatoxin M1 (AFM1) in milk and milk powders is described. A monoclonal antibody and an immobilized protein–AFM1 conjugate are utilized in a simple inhibition format following aqueous extraction and immunoaffinity clean-up of the sample, thereby avoiding the need for signal amplification techniques. The sensor surface is stable over multiple regeneration cycles, and the technique yields a method detection limit of 0.1 ng g−1, which is five times lower than the European Commission maximum residue limit. The described antibody-based biosensor technique provides the advantages of quantitative data, automation, and real-time and non-labeled detection of AFM1. The method therefore facilitates routine quantitative threshold-level screening for the identification of potential non-compliance of AFM1 content prior to confirmatory analysis by reference chromatographic methods and may be considered to complement the enzyme-linked immunosorbent assay technique.

Keywords

Aflatoxin M1 Milk Biosensor Surface plasmon resonance Immunoassay 

Notes

Acknowledgments

The authors acknowledge Dr. Terry Cooney (Analytica Laboratories Ltd., Hamilton, New Zealand) for LC–tandem mass spectrometry analyses and the support and encouragement of Dr. Robert Crawford and Fonterra Co-operative Group Ltd. throughout this study.

Compliance with Ethical Standards

Conflict of Interest

Harvey Indyk declares that he has no conflict of interest. Sowmya Chetikam declares that she has no conflict of interest. Brendon Gill declares that he has no conflict of interest. Jackie Wood declares that she has no conflict of interest. David Woollard declares that he has no conflict of interest.

Ethical Approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed Consent

Not applicable.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Fonterra Co-operative Group LtdWaitoaNew Zealand
  2. 2.Hill LaboratoriesHamiltonNew Zealand

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