Rapid Extraction of Polycyclic Aromatic Hydrocarbons in Apple: Ultrasound-Assisted Solvent Extraction Followed by Microextraction by Packed Sorbent
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Common procedures for polycyclic aromatic hydrocarbons (PAHs) extraction involve the use of large amount of solvents and a long extraction time. To address these difficulties, ultrasound-assisted solvent extraction followed by microextraction by packed sorbent (UAE-MEPS) was investigated as a novel extraction procedure for the recovery of PAHs from apple, a model of solid vegetal food matrix. The most suitable sorbent and the eluting solvent for PAHs recovery through MEPS were determined. The performances of the whole procedure were tested through the determination of linearity, accuracy, and sensitivity after GC-MS analyses. Linearity assays, within 0 to 10 μg/kg in apple (9 points), provided determination coefficients around 0.993 (median value) for all studied PAHs. Repeatability tests (n = 3) and intermediate precision (n = 9) showed relative standard deviation between 1 and 12%. Limits of detection were determined between 0.04 and 0.13 μg/kg wet weight (w.w.) and limits of quantification were between 0.12 and 0.43 μg/kg w.w. These performances allowed the trace level determination of PAHs in apples collected in different crop environments. The proposed UAE-MEPS procedure is rapid and few solvent consuming compared to other conventional techniques for PAHs extraction.
KeywordsPolycyclic aromatic hydrocarbons Apple Ultrasound-assisted solvent extraction Microextraction by packed sorbent GC-MS
Ultrasound-assisted solvent extraction
Microextraction by packed sorbent
Polycyclic aromatic hydrocarbons
Molecular imprinted polymers
Pressurized liquid extraction
Headspace solid-phase microextraction
Single ion monitoring
Limit of detection
Limit of quantification
Porous graphitic carbon
Relative standard deviation
Alice Paris was supported by a PhD scholarship from the Basse-Normandie Region.
Compliance with Ethical Standards
Conflict of Interest
Alice Paris declares that she has no conflict of interest. Jean-Luc Gaillard declares that he has no conflict of interest. Jérôme Ledauphin declares that he has no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors
A statement regarding informed consent is not applicable.
- Abdel-Rehim M (2004) New trend in sample preparation: on-line microextraction in packed syringe for liquid and gas chromatography applications. I. Determination of local anaesthetics in human plasma samples using gas chromatography-mass spectrometry. J Chromatogr B 801:317–321. https://doi.org/10.1016/j.jchromb.2003.11.042 CrossRefGoogle Scholar
- Abou-Arab AAK, Abou-Donia MAM, El-Dars FMSE, Ali OIM, Hossam AG (2014) Levels of polycyclic aromatic hydrocarbons (PAHS) in some Egyptian vegetables and fruits and their influences by some treatments. Int J Curr Microbiol App Sci 3:277–293Google Scholar
- Bishnoi NR, Mehta U, Pandit GG (2006) Quantification of polycyclic aromatic hydrocarbons in fruits and vegetables using high performance liquid chromatography. Indian J Chem Technol 13:30–35Google Scholar
- El-Baqqali A, Kussak A, Abdel-Rehim M (2006) Fast and sensitive environmental analysis utilizing microextraction in packed syringe online with gas chromatography-mass spectrometry determination of polycyclic aromatic hydrocarbons in water. J Chromatogr A 1114:234–238. https://doi.org/10.1016/j.chroma.2006.02.024 CrossRefGoogle Scholar
- Haider W, Barillier D, Hayat A, Gaillard JL, Ledauphin J (2014) Rapid quantification and comparison of major volatile compounds of ciders from France (Normandy and Brittany) using microextraction by packed sorbent (MEPS). Anal Methods 6:1364–1376. https://doi.org/10.1039/C3AY41385C CrossRefGoogle Scholar
- Martínez-Moral MP, Tena M (2014) Use of microextraction by packed sorbents following selective pressurized liquid extraction for the determination of brominated diphenyl ethers in sewage sludge by gas chromatography-mass spectrometry. J Chromatogr A 1364:28–35. https://doi.org/10.1016/j.chroma.2014.08.075 CrossRefGoogle Scholar
- Quinto M, Amodio P, Spadaccino G, Centonze D (2012) Development of a mathematical model online microextraction by packed sorbent under equilibrium conditions and its application for polycyclic aromatic hydrocarbon determination in water by gas chromatography-mass spectrometry. J Chromatogr A 1262:12–26. https://doi.org/10.1016/j.chroma.2012.08.098 CrossRefGoogle Scholar
- Sánchez MN, Santos PM, Sappó CP, Pavón JLP, Cordero BM (2014) Microextraction by packed sorbent and salting-out-assisted liquid-liquid extraction for the determination of aromatic amines formed from azo dyes in textiles. Talanta 119:375–384. https://doi.org/10.1016/j.talanta.2013.11.041 CrossRefGoogle Scholar