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Monolithic Material Prepared with Nanodiamond as Monomer for the Enrichment of β-Sitosterol in Edible Oil

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Abstract

In this paper, a hybrid monolithic column with modified nanodiamond and 1-dodecene as co-monomers was prepared and applied to the online enrichment and purification of β-sitosterol in edible oil. The obtained monolithic column was characterized by scanning electron microscopy (SEM) and nitrogen adsorption–desorption isotherm measurement, which indicated that the monolith possessed characteristics of porous structure and high permeability. Under the optimum conditions for extraction and determination, the linear regression coefficient for β-sitosterol was 0.998; the limit of detection (LOD) and the limit of quantitation (LOQ) were 3.0 μg mL−1 and 10.0 μg mL−1, respectively; the relative standard deviation (RSD) of intra-day and inter-day assays for four edible oils were 2.33–6.58% and 3.24–6.94%, respectively. The recovery was in the range of 89.6–105.5%. The results showed that the hybrid monolithic column with high selectivity and good permeability were successfully used as online solid-phase extraction (SPE) column for determination of β-sitosterol in edible oil.

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Funding

The work was supported by the National Natural Science Foundation of China (nos. 21575033, 21505030).

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Authors and Affiliations

  1. Key Laboratory of Pharmaceutical Quality Control of Hebei Province, College of Pharmaceutical Sciences, Hebei University, Baoding, 071002, China

    Beijiao Cui, Haiyan Liu, Huan Yu, Xiaoya Pang & Ligai Bai

  2. Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Baoding, 071002, China

    Beijiao Cui, Haiyan Liu, Huan Yu, Xiaoya Pang, Hongyuan Yan & Ligai Bai

  3. College of Public Health, Hebei University, Baoding, 071002, China

    Hongyuan Yan

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  1. Beijiao Cui
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  2. Haiyan Liu
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  3. Huan Yu
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  4. Xiaoya Pang
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  5. Hongyuan Yan
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Correspondence to Haiyan Liu or Ligai Bai.

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Beijiao Cui declares that he has no conflict of interest. Huan Yu declares that he has no conflict of interest. Xiaoya Pang declares that he has no conflict of interest. Hongyuan Yan declares that he has no conflict of interest. Ligai Bai declares that he has no conflict of interest.

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Cui, B., Liu, H., Yu, H. et al. Monolithic Material Prepared with Nanodiamond as Monomer for the Enrichment of β-Sitosterol in Edible Oil. Food Anal. Methods 12, 697–704 (2019). https://doi.org/10.1007/s12161-018-1405-9

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  • DOI: https://doi.org/10.1007/s12161-018-1405-9

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