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A Turn-on Biosensor-Based Aptamer-Mediated Carbon Quantum Dots Nanoaggregate for Acetamiprid Detection in Complex Samples

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Abstract

In this paper, we developed a new biosensor-based on aptamer-mediated carbon quantum dots (apt-CQDs) nanoaggregate for acetamiprid detection. The acetamiprid aptamer, 5′- and its complementary DNA were labeled on CQDs, respectively. The hybridization of aptamer and its complementary DNA triggered the formation of nanoaggregate of CQDs, leading to the fluorescence quenching of the CQDs. Upon addition of acetamiprid, specific recognition of acetamiprid caused the release of the cDNA-CQDs and the fluorescence intensity recovered which was linearly related to the concentration of acetamiprid. The sensor provided a linear range from 0.2 to 20 ng/L for acetamiprid with a detection limit of 0.04 ng/L in an aqueous buffer. The same linear range was obtained in spiked human serum samples and lettuce samples with similar detection limits (0.15 and 0.11 ng/L), demonstrating high robustness of the sensor in a complex sample matrix. The as-prepared biosensor was used to monitor acetamiprid level in real samples with recovery ranged from 86 to 107%, and RSDs ranged from 1.3–6.9% (n = 3). The proposed study not only provided a simple method through CQDs nanoaggregate and also provided a turn-on detection mode for acetamiprid in real samples with high signal-to-noise ratios.

Fabrication of CQDs and principle for detection of acetamiprid by CQDs nanoaggregates.

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Funding

This work was supported by the National Natural Science Foundation of China (21305013) and the Guangdong Provincial Key Platform and Major Scientific Research Projects for Colleges and Universities (Nos. 2014KZDXM073, 2015KCXTD029).

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Author notes

  1. Zhe Jiao and Hongfeng Zhang contributed equally to this work.

Authors and Affiliations

  1. School of Environment and Civil Engineering, Dongguan University of Technology, Dongguan, 523808, China

    Zhe Jiao & Dan Zhu

  2. Guangzhou Center for Disease Control and Prevention, Guangzhou, 510440, China

    Hongfeng Zhang

  3. Tobacco Science Institute of Jiangxi Province, Nanchang, 330025, China

    Shaohe Jiao

  4. Integrated Technology Center of Dongguan Entry-Exit Inspection and Quarantine Bureau, Dongguan, 523073, China

    Zongning Guo

  5. School of Electrical Engineering and Intelligentization, Dongguan University of Technology, Dongguan, 523808, China

    Xiaofang Zhao

Authors
  1. Zhe Jiao
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  2. Hongfeng Zhang
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  3. Shaohe Jiao
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  4. Zongning Guo
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  5. Dan Zhu
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Contributions

Hongfeng Zhang contributed to the independent LC-MS/MS method for acetamiprid detection.

Corresponding author

Correspondence to Zhe Jiao.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Conflict of Interest

Zhe Jiao declares that she/he has no conflict of interest. Hongfeng Zhang declares that she/he has no conflict of interest. Shaohe Jiao declares that she/he has no conflict of interest. Zongning Guo declares that she/he has no conflict of interest. Dan Zhu declares that she/he has no conflict of interest. Xiaofang Zhao declares that she/he has no conflict of interest.

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Jiao, Z., Zhang, H., Jiao, S. et al. A Turn-on Biosensor-Based Aptamer-Mediated Carbon Quantum Dots Nanoaggregate for Acetamiprid Detection in Complex Samples. Food Anal. Methods 12, 668–676 (2019). https://doi.org/10.1007/s12161-018-1393-9

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  • DOI: https://doi.org/10.1007/s12161-018-1393-9

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