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Determination of Biogenic Amines in Fish Meat and Fermented Foods Using Column-Switching High-Performance Liquid Chromatography with Fluorescence Detection

Abstract

In this study, we developed a column-switching high-performance liquid chromatography method with fluorescence detection for the simultaneous analysis of biogenic amines (BAs). This method uses an isocratic solution using acetonitrile with water as the mobile phase. Column-switching is achieved by using a switching valve with a set time program to change flow direction. Using this method, seven BAs (tryptamine, 2-phenylethylamine, putrescine, cadaverine, histamine, tyramine, and spermidine) could be separated and impurities eliminated. Using fluorescence detection, BAs could be identified with high sensitivity. We employed this method to determine the BA contents of fish (mackerel, tuna, and cod) and fermented food (a soybean product and cheese). Our results are in line with previous reports, yielded highly reproducible and quantitative results, and enabled the quick and simultaneous analysis of multiple BAs. Therefore, the method developed here may be useful for the continuous analysis of BAs in different samples to evaluate food quality.

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Funding

This work was supported by the Grant-in-Aid for JSPS Research Fellow Grant Number 17J01852.

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Correspondence to Ryusuke Tanaka.

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Conflict of Interest

Mami Ishimaru declares that she has no conflict of interest. Yuma Muto declares that he has no conflict of interest. Akari Nakayama declares that she has no conflict of interest. Hideo Hatate declares that he has no conflict of interest. Ryusuke Tanaka declares that he has no conflict of interest.

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Ishimaru, M., Muto, Y., Nakayama, A. et al. Determination of Biogenic Amines in Fish Meat and Fermented Foods Using Column-Switching High-Performance Liquid Chromatography with Fluorescence Detection. Food Anal. Methods 12, 166–175 (2019). https://doi.org/10.1007/s12161-018-1349-0

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  • DOI: https://doi.org/10.1007/s12161-018-1349-0

Keywords

  • Biogenic amines
  • Column-switching HPLC
  • Fluorescence detection
  • Fish
  • Fermented food