Analytical Approach for Detection of Ergosterol in Mushrooms Based on Modification Free Electrochemical Sensor in Organic Solvents
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Ergosterol (Erg) is the major sterol component in the plasma membranes of fungi. The main goal of this work was to develop rapid, reliable, and cost-effective method toward Erg detection based on application of unmodified boron-doped diamond electrode. Several organic solvents were tested (acetonitrile, dimethyformamide, dimethylsulfoxide, dichloromethane), and it was found that best analytical characteristics were achieved using acetonitrile containing 0.1 M tetrabutylammonium hexa-fluorophosphate as supporting electrolyte. Oval shaped and well-defined oxidation peak was recorded at potential of around 1.17 V versus Ag/Ag+ non-aqueous reference electrode. Under optimized experimental conditions, linear working range from 2 to 200 μM was estimated, with limit of detection of 0.7 μM, using square wave voltammetry. Negligible effect of possible interfering compound was observed. Proposed methodology was successfully applied for estimation of Erg content in mushrooms extract with satisfactory results in comparison with spectrophotometric procedure. Obtained results clearly show that developed analytical methodology can be adequate replacement for the, up to date, used methods and open-novel approach for ergosterol detection.
KeywordsErgosterol Boron-doped diamond electrode Mushrooms
This work was supported by Magbiovin project (FP7-ERAChairs-Pilot Call-2013, Grant agreement: 621375), by the Ministry of Education, Science and Technology, the Republic of Serbia (Project No. OI 172030), and the Grant Agency of the Slovak Republic (Grant No. 1/0489/16).
Compliance with Ethical Standards
Conflict of Interest
Vesna Vukojević declares that she has no conflict of interest. Sladjana Djurdjić declares that she has no conflict of interest. Ľubomír Švorc declares that he has no conflict of interest. Tanja Ćirković-Veličković declares that she has no conflict of interest. Jelena Mutić declares that she has no conflict of interest. Dalibor M. Stanković declares that he has no conflict of interest.
This article does not contain any studies with human or animal subjects performed by any of the authors.
Informed consent is not applicable for the nature of this study.
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