One-Step Core/Multishell Quantum Dots-Based Fluoroimmunoassay for Screening of Deoxynivalenol in Maize
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Deoxynivalenol (DON) is a protein synthesis inhibitor produced by the Fusarium species, which frequently contaminates grains used for human or animal consumption, thereby necessitating their effective monitoring and control. Antibody-based immunoassays provide efficient and cheap methods for screening this mycotoxin. To achieve high sensitivity and avoid enzymatic reaction, a new highly sensitive monoclonal antibody (MAb) and a fluorescent probe consisting of amino-functionalized core/multishell quantum dots (QDs), CdSe/CdS/Cd0.5Zn0.5S/ZnS, bio conjugated to 3-HS-DON-BSA were prepared; then, a sensitive and reliable direct competitive fluorescent-labeled immunosorbent assay (dcFLISA) was developed. Under optimized conditions, the 50% inhibition concentration (IC50) value of the dcFLISA was 5.6 μg/kg with limit of detection of 12.2 μg/kg in maize samples. The recoveries ranged from 77.3 to 84.3% with coefficients of variation (CV%) values below 10%. Good correlation between the dcFLISA and HPLC-MS/MS results in the naturally contaminated maize demonstrated that the dcFLISA was a rapid and sensitive with high-performance and reliable method for the detection of DON. The potential of this method to function as a simple and efficient strategy for small molecule on-site screening might be applied in the future.
KeywordsMonoclonal antibody Deoxynivalenol Quantum dots Direct competitive fluorescent-labeled immunosorbent assay
This work was supported by grants from Natural Science Foundation of China (U1301214 and 31502114) and Special Fund for Agro-scientific Research in the Public Interest (201203040). And we are very thankful to Prof. Sarah De Saeger (Laboratory of Food Analysis, Faculty of Pharmaceutical Sciences, Ghent University) for providing naturally contaminated maize samples.
Xiya Zhang and Xuezhi Yu contributed equally to this paper.
Compliance with Ethical Standards
Conflict of Interest
Xiya Zhang declares that he has no conflict of interest. Xuezhi Yu declares that she has no conflict of interest. Jianyi Wang declares that he has no conflict of interest. Qi wang declares that she has no conflict of interest. Hui Meng declares that he has no conflict of interest. Zhanhui Wang declares that he has no conflict of interest.
This article does not contain any studies with human subjects. All animal experiments described in this study were approved by the Animal Care Center of the China Agricultural University, Beijing, China, following all institutional and national guidelines for the care and use of laboratory animals.
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