Rapid Analysis of Bitertanol in Agro-products Using Molecularly Imprinted Polymers-Surface-Enhanced Raman Spectroscopy
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In this work, we first reported molecularly imprinted polymers-surface-enhanced Raman spectroscopy (MIP-SERS) for rapid detection and quantification of bitertanol in cucumber and peaches. In order to remove interference-mitigation effects and avoid template leakage, MIPs were successfully synthesized based on triadimefon molecules as the dummy template, methacrylic acid (MAA) as a functional monomer, trimethylolpropane trimethacrylate (TRIM) as a cross-linker, and 2,2-azobis-isobutyronitrile (AIBN) as an initiator. Static adsorption experiments and Scatchard analysis were then conducted and results showed that the synthesized MIPs could rapidly and selectively adsorb and separate bitertanol from cucumber and peaches due to their predetermined recognition sites. The capacity of MIPs for absorbing bitertanol (∼ 2.21 mg/g) was approximately 1.5 times that of non-imprinted polymers (NIPs) (∼ 1.55 mg/g). Gold nanoparticles (AuNPs) synthesized by sodium citrate reduction method were validated as a suitable SERS colloid for enhancing Raman signals. SERS spectral peaks (760, 985, 1190, and 1279 cm−1) were used to develop quantitative analysis based on partial least-squares regression (PLSR) for bitertanol in cucumber and peaches. The LODs for this method were 0.041 and 0.029 mg/kg in cucumber and peaches, respectively. The entire analysis process required 15 min or less. More importantly, the MIP-SERS system provided not only a rapid, sensitive, and reliable method for bitertanol detection, but also a routine for overcoming the interference-mitigation effects in the SERS technology.
KeywordsMIP-SERS Bitertanol Principal component analysis (PCA)
Financial support from the Special Fund for Agro-Scientific Research in the Public Interest of China (201209094), National Natural Science Foundation of China (Contract No. 31471654), and National Key Technology R&D Program for the 12th five-year plan (2014BAD13B05–05) are greatly appreciated.
Compliance with Ethical Standards
Conflict of Interest
Xiaolin Cao declares that he has no conflict of interest. Fengnian Zhao declares that he has no conflict of interest. Zejun Jiang declares that he has no conflict of interest. Sihui Hong declares that she has no conflict of interest. Chao Zhang declares that she has no conflict of interest. Yongxin She declares that he has no conflict of interest. Fen Jin declares that he has no conflict of interest. Maojun Jin declares that he has no conflict of interest. Jing Wang declares that she has no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
Informed consent is not applicable in this study.
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