Food Analytical Methods

, Volume 11, Issue 5, pp 1303–1311 | Cite as

Simultaneous Determination of Aflatoxin B1, Bisphenol A, and 4-Nonylphenol in Peanut Oils by Liquid-Liquid Extraction Combined with Solid-Phase Extraction and Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry

  • Hongling Deng
  • Xinguo Su
  • Haibo Wang


An analytical method based on liquid-liquid extraction combined with solid-phase extraction and isotope dilution-ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was well developed for simultaneous determination of aflatoxin B1 (AFB1), bisphenol A (BPA), and 4-nonylphenol (4-NP) in peanut oil. After adding isotope internal standards, the samples were firstly diluted by normal hexane and then extracted by acetonitrile and Carb/PSA solid-phase extraction cartridge in sequence to obtain the extracted solution. All the extracted solution was merged and was subsequently dried to near dryness by a mild nitrogen stream. Three target analytes were separated on a Phenomenex Luna C18 chromatographic column, quantified by an internal standard method and detected by ESI positive (ESI+) and negative (ESI) subsection acquisition modes under multi-reaction monitoring (MRM) conditions. Results demonstrated that the three target analytes exhibited excellent linearity in their corresponding concentration ranges of 0.1–100.0 μg/L with correlation coefficients all greater than 0.998. The corresponding method limits of quantitation (MLOQ, S/N = 10) of AFB1, BPA, and 4-NP were 0.2, 1.0, and 2.0 μg/kg, respectively. Moreover, the mean recoveries for negative samples spiked at three concentration levels were calculated between 87.7 and 105.1% with relative standard deviation (RSD, n = 6) ranging from 2.2 to 7.9% and the interday precision (n = 5) ranging from 5.0 to 8.7%. Finally, the method was successfully applied to analyze 52 peanut oil samples, and AFB1 and 4-NP were detected in 43 samples with the concentrations in the ranges of 0.5–69.4 and 9.3–77.8 μg/kg, respectively. None of BPA was detected in any samples.


Peanut oil Aflatoxin B1 Bisphenol A 4-Nonylphenol Solid-phase extraction Liquid chromatography-tandem mass spectrometry (LC-MS/MS) 


Compliance with Ethical Standards

Conflict of Interest

Hongling Deng declares that he has no conflict of interest. Xinguo Su declares that he has no conflict of interest. Haibo Wang declares that she has no conflict of interest.

Ethical Approval

This article does not contain any studies with animals performed by any of the authors.

Informed Consent

Not applicable.

Supplementary material

12161_2017_1113_MOESM1_ESM.docx (26 kb)
ESM 1 (DOCX 26.3 kb)
12161_2017_1113_MOESM2_ESM.docx (16 kb)
ESM 2 (DOCX 16.0 kb)


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2017

Authors and Affiliations

  1. 1.Guangdong Food and Drug Vocational CollegeGuangzhouChina

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