Abstract
Differential scanning calorimetry (DSC) was used to study the thermal behavior of Colombian honeys produced by the honey-bee Apis mellifera and by three species of stingless bees: Melipona fuscipes, Melipona favosa favosa, and Melipona compressipes. The honey samples were collected every 2 months over the course of year (August 2014 to August 2015). Up to four thermal transitions (trs1–trs4a, trs4b) were found in the honey samples: M. fuscipes (trs1–trs4a), M. favosa (trs1–trs4a, trs4b), M. compressipes (trs1, trs3, trs4a), and A. mellifera (trs1, trs3, trs4a). Statistical analyses showed significant differences in enthalpies of each transition between species; therefore, DSC analysis can be used as a finger-print to differentiate the honeys of species.








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Ahmed ST, Prabhu GS, Raghavan V, Ngadi M (2007) Physico-chemical, rheological, calorimetric and dielectric behavior of selected Indian honey. J Food Eng 79(4):1207–1213. https://doi.org/10.1016/j.jfoodeng.2006.04.048
Bentabol M, Hernández AZ, Rodríguez B, Rodríguez E, Díaz C (2011) Differentiation of blossom and honeydew honeys using multivariate analysis on the physicochemical parameters and sugar composition. Food Chem 126(2):664–672. https://doi.org/10.1016/j.foodchem.2010.11.003
Bentabol M, Hernández AZ, Rodríguez B, Rodríguez E, Díaz C (2014) Physicochemical characteristics of minor monofloral honeys from Tenerife, Spain. Food Sci Technol 55(2):572–578. https://doi.org/10.1016/j.lwt.2013.09.024
Bijlsma L, De Bruijn LM, Martens E, Sommeijer M (2006) Water content of stingless bee honeys (Apidae, Meliponini): interspecific variation and comparison with honey of Apis mellifera. Apidologie 37:480–486
Bogdanov S, Vit P, Kilchenmann V (1996) Sugar profiles and conductivity of stingless bee honeys from Venezuela. Apidologie 27(6):445–450
Bruylants G, Wouters J, Michaux C (2005) Differential scanning calorimetry in life science: thermodynamics, stability, molecular recognition and application in drug design. Curr Med Chem 12(17):2011–2020
Can Z, Yildiz O, Sahin H, Turumtay EA, Silici S, Kolayli S (2015) An investigation of Turkish honeys: their physico-chemical properties, antioxidant capacities and phenolic profiles. Food Chem 180:133–141. https://doi.org/10.1016/j.foodchem.2015.02.024
Cordella C, Antinelli JF, Aurieres C, Faucon JP, Cabrol-Bass D, Sbirrazzuoli N (2002) Use of differential scanning calorimetry (DSC) as a new technique for detection of adulteration in honeys. 1. Study of adulteration effect on honey thermal behavior. J Agric Food Chem 50(1):203–208
Chakir A, Romane A, Marcazzan GL, Ferrazzi P (2011) Physicochemical properties of some honeys produced from different plants in Morocco. Arab J Chem. https://doi.org/10.1016/j.arabjc.2011.10.013
Dardón MJ, Maldonado C, Enríquez E (2013) “The pot-honey of Guatemalan bees.” In pot honey, edited by Patricia Vit, Silvia R. M. Pedro and David Roubik, 395-408. Springer New York
de Almeida-Muradian, Ligia Bicudo (2013) “Tetragonisca angustula pot-honey compared to Apis mellifera honey from Brazil.” In pot-honey, edited by Patricia Vit, Silvia R. M. Pedro and David Roubik, 375–382. Springer New York
Fahim H, Dasti JI, Ihsan A, Ahmed S, Nadeem M (2014) Physico-chemical analysis and antimicrobial potential of Apis dorsata, Apis mellifera and Ziziphus jujube honey samples from Pakistan. Asian Pac J Trop Biomed 4(8):633–641. https://doi.org/10.12980/APJTB.4.2014APJTB-2014-0095
Fuenmayor CA, Díaz AC, Zuluaga CM, Quicazán MC (2013) Honey of Colombian stingless bees: nutritional characteristics and physicochemical quality indicators. In: pot-honey, edited by Patricia Vit, Silvia R. M. Pedro and David Roubik. Springer New York, pp 383–394
Gianelli MP, Ponce MC, Venegas C (2010) Volatile compounds in honey produced in the Central Valley of Ñuble Province, Chile. Chil J Agric Res 70:75–84
Guerrini A, Bruni R, Maietti S, Poli F, Rossi D, Paganetto G, Muzzoli M, Scalvenzi L, Sacchetti G (2009) Ecuadorian stingless bee (Meliponinae) honey: a chemical and functional profile of an ancient health product. Food Chem 114(4):1413–1420. https://doi.org/10.1016/j.foodchem.2008.11.023
Hurtta M, Pitkänen I, Knuutinen J (2004) Melting behaviour of D-sucrose, D-glucose and D-fructose. Carbohydr Res 339(13):2267–2273. https://doi.org/10.1016/j.carres.2004.06.022
Johnson CM (2013) Differential scanning calorimetry as a tool for protein folding and stability. Arch Biochem Biophys 531(1–2):100–109. https://doi.org/10.1016/j.abb.2012.09.008
Kántor Z, Pitsi G, Thoen J (1999) Glass transition temperature of honey as a function of water content as determined by differential scanning calorimetry. J Agric Food Chem 47(6):2327–2330. https://doi.org/10.1021/jf981070g
Lazarević KB, Andrić F, Trifković J, Tešić Ž, Dušanka M (2012) Characterisation of Serbian unifloral honeys according to their physicochemical parameters. Food Chem 132(4):2060–2064. https://doi.org/10.1016/j.foodchem.2011.12.048
Lazaridou A, Biliaderis CG, Bacandritsos N, Sabatini AG (2004) Composition, thermal and rheological behaviour of selected Greek honeys. J Food Eng 64(1):9–21. https://doi.org/10.1016/j.jfoodeng.2003.09.007
Lee JW, Thomas LC, Shelly JS (2011) Investigation of the heating rate dependency associated with the loss of crystalline structure in sucrose, glucose, and fructose using a thermal analysis approach (part I). J Agr Food Chem 59(2):684–701. https://doi.org/10.1021/jf1042344
Lupano CE (1997) DSC study of honey granulation stored at various temperatures. Food Res Int 30(9):683–688. https://doi.org/10.1016/S0963-9969(98)00030-1
Lyubarev AE, Kurganov BI (2000) Analysis of DSC data relating to proteins undergoing irreversible thermal denaturation. J Therm Anal Calorim 62(1):51–62. https://doi.org/10.1023/A:1010102525964
Martinez JC, Viguera AR, Serrano L, Filimonov VV, Mateo PL (1998) The DSC data analysis for small, single-domain proteins. Application to the SH3 domain. Reac Func Polym 36(3):221–225. https://doi.org/10.1016/S1381-5148(97)00088-6
Michener CD (2007) “The importance of bees.” In The bees of the world, 953. Baltimore: Johns Hopkins University Press
Mizuno M, Pikal MJ (2013) Is the pre-Tg DSC endotherm observed with solid state proteins associated with the protein internal dynamics? Investigation of bovine serum albumin by solid state hydrogen/deuterium exchange. Eur J Pharm Biopharm 85(2):170–176. https://doi.org/10.1016/j.ejpb.2013.04.019
Özcan MM, Ölmez Ç (2014) Some qualitative properties of different monofloral honeys. Food Chem 163:212–218. https://doi.org/10.1016/j.foodchem.2014.04.072
Pasini F, Gardini S, Marcazzan GL, Caboni MF (2013) Buckwheat honeys: screening of composition and properties. Food Chem 141(3):2802–2811. https://doi.org/10.1016/j.foodchem.2013.05.102
Perez R, Sanchez AC, Calvo RM, Tadeo JL (2002) Analysis of volatiles from Spanish honeys by solid-phase microextraction and gas chromatography-mass spectrometry. J Agric Food Chem 50(9):2633–2637
Piasenzotto L, Gracco L, Conte L (2003) Solid phase microextraction (SPME) applied to honey quality control. J Sci Food Agr 83(10):1037–1044. https://doi.org/10.1002/jsfa.1502
Rojas-Aguilar A, Ginez-Carbajal F, Orozco-Guareńo E, Flores-Segura H (2005) Measurement of enthalpies of vaporization of volatile heterocyclic compounds by DSC. J Therm Anal Calorim 79(1):95–100. https://doi.org/10.1007/s10973-004-0568-3
Serra BJ, Coll FV (2003) Flavour index and aroma profiles of fresh and processed honeys. J. Sci. Food Agr. 83(4):275–282. https://doi.org/10.1002/jsfa.1308
Skoog DA, West DM, Holler FJ (1997) Fundamentos de química analítica. 2 ed. Vol. 2
Soto VC, Maldonado IB, Jofré VP, Galmarini CR, Silva MF (2015) Direct analysis of nectar and floral volatile organic compounds in hybrid onions by HS-SPME/GC–MS: relationship with pollination and seed production. Microchem J 122:110–118. https://doi.org/10.1016/j.microc.2015.04.017
Tornuk F, Karaman S, Ozturk I, Toker OS, Tastemur B, Sagdic O, Dogan M, Kayacier A (2013) Quality characterization of artisanal and retail Turkish blossom honeys: determination of physicochemical, microbiological, bioactive properties and aroma profile. Ind Crop Prod 46:124–131. https://doi.org/10.1016/j.indcrop.2012.12.042
Torres A, Hoffmann W, Lamprecht I (2007) Thermal investigations of a nest of the stingless bee Tetragonisca angustula Illiger in Colombia. Thermochim Acta 458(1–2):118–123. https://doi.org/10.1016/j.tca.2007.01.024
Torres A, Garedew A, Schmolz E, Lamprecht I (2004) Calorimetric investigation of the antimicrobial action and insight into the chemical properties of “angelita” honey—a product of the stingless bee Tetragonisca angustula from Colombia. Thermochim Acta 415(1–2):107–113. https://doi.org/10.1016/j.tca.2003.06.005
Venir E, Spaziani E, Maltini E (2010) Crystallization in “Tarassaco” Italian honey studied by DSC. Food Chem 122(2):410–415. https://doi.org/10.1016/j.foodchem.2009.04.012
Vit P (2013) Melipona favosa pot-honey from Venezuela. In: pot honey, edited by Patricia Vit, Silvia R. M. Pedro and David Roubik. Springer, New York pp. 363–373
Vit P, Oddo L, Persano M, Marano L, Salas de Mejias E (1998) Venezuelan stingless bee honeys characterized by multivariate analysis of physicochemical properties. Apidologie 29(5):377–389
Vit P, Vargas O, López T, Maza F (2015) Meliponini biodiversity and medicinal uses of pot-honey from El Oro province in Ecuador. Emir J Food Agric 27(6):502–506
Wedmore E (1995) The accurate determination of the water content of honeys. Bee World 36:197–206
Wen J, Arthur K, Chemmalil L, Muzammil S, Gabrielson J, Jiang Y (2012) Applications of differential scanning calorimetry for thermal stability analysis of proteins: qualification of DSC. J Pharm Sci 101(3):955–964. https://doi.org/10.1002/jps.22820
Wolski T, Tambor K, Rybak H, BogdanKêdzia (2006) Identification of honey volatile components by solid phase microextraction (SPME) and gas chromatography/mass spectrometry (GC/MS). J Apc Sci 50 (2):115–126
Yücel Y, ˘lu Sultanog P (2013) Characterization of honeys from Hatay Region by their physicochemical properties combined with chemometrics. Food Bioscience 1:16–25. https://doi.org/10.1016/j.fbio.2013.02.001
Yutaka I (1991) Thermal denaturation of proteins II. Hitachi High-Technologies. Available from http://www.hitachi-hightech.com/global/
Zhou J, Suo Z, Zhao P, Cheng N, Gao H, Zhao J, Cao W (2013) Jujube honey from China: physicochemical characteristics and mineral contents. J Food Sci 78(3):C387–C394. https://doi.org/10.1111/1750-3841.12049
Acknowledgements
This work was supported by Ricola Foundation (Switzerland), within the framework of the project “Stingless bees as alternative pollinators” and the Universidad de Pamplona, Vicerrectoría de Investigaciones.
Funding
This study was funded by Ricola Foundation (Switzerland) and Universidad de Pamplona (grant number NI576).
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Author Yaneth Cardona declares that she has no conflict of interest. Author Alexandra Torres declares that she has no conflict of interest. Author Wolfgang Hoffmann declares that he has no conflict of interest. Author Ingolf Lamprecht declares that he has no conflict of interest.
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Cardona, Y., Torres, A., Hoffmann, W. et al. Differentiation of Honey from Melipona Species Using Differential Scanning Calorimetry. Food Anal. Methods 11, 1056–1067 (2018). https://doi.org/10.1007/s12161-017-1083-z
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DOI: https://doi.org/10.1007/s12161-017-1083-z


