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Determination of Trans-resveratrol in Wines, Spirits, and Grape Juices Using Solid-Phase Micro Extraction Coupled to Liquid Chromatography with UV Diode-Array Detection

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Abstract

Solid phase microextraction (polyacrylate fiber), coupled to liquid chromatography with UV diode-array detection, has been optimized for the determination of trans-resveratrol in wines, spirits, and grape juices. The main aspects influencing fiber adsorption (fiber coating, extraction time, ethanol content, salt addition) and desorption (desorption and injection time, desorption solvent mixture composition, carryover) of the analyte have been investigated. The method permitted a fast and simple determination of free trans-resveratrol in commercial samples. It was found in all the analyzed samples at concentration levels ranging from 0.007 to 4.486 μg mL−1. Total trans-resveratrol concentrations were also evaluated after enzymatic deconjugation of piceid.

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Authors and Affiliations

  1. Department of Chemistry, University of Bari “Aldo Moro”, Via Orabona 4, 70125, Bari, Italy

    Antonella Aresta, Federica Massari & Carlo Zambonin

  2. CONISMA, Piazzale Flaminio 9, 00196, Rome, Italy

    Pietro Cotugno

Authors
  1. Antonella Aresta
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  2. Pietro Cotugno
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  3. Federica Massari
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  4. Carlo Zambonin
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Correspondence to Antonella Aresta.

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Funding

This project was financed by Università degli Studi di Bari “Aldo Moro”.

Conflict of Interest

Antonella Aresta declares that she has no conflict of interest. Pietro Cotugno declares that he has no conflict of interest. Federica Massari declares that she has no conflict of interest. Carlo Zambonin declares that he has no conflict of interest. This article does not contain any studies with human or animal subjects.

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In this study, humans are not involved.

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Aresta, A., Cotugno, P., Massari, F. et al. Determination of Trans-resveratrol in Wines, Spirits, and Grape Juices Using Solid-Phase Micro Extraction Coupled to Liquid Chromatography with UV Diode-Array Detection. Food Anal. Methods 11, 426–431 (2018). https://doi.org/10.1007/s12161-017-1013-0

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  • DOI: https://doi.org/10.1007/s12161-017-1013-0

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