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Food Analytical Methods

, Volume 10, Issue 11, pp 3647–3657 | Cite as

Evaluation of a Selective Approach for the Determination of 5-Nitroimidazoles in Aquaculture Products by Capillary Liquid Chromatography Using Molecularly Imprinted Solid-Phase Extraction

  • Maykel Hernández-Mesa
  • David Moreno-González
  • Carmen Cruces-Blanco
  • Ana M. García-Campaña
Article

Abstract

Capillary liquid chromatography with UV detection is proposed for the determination of 5-nitroimidazole residues in aquaculture products. The separation was carried out in a C18 column at 20 °C, using a mobile phase consisting of water and acetonitrile, at 7 μL/min and 320 nm as detection wavelength. Furthermore, full loop injection mode (8 μL) was selected and water was considered as injection solvent. The optimized method was applied to the monitoring of nine 5-nitroimidazoles, including three metabolites, in crab, salmon, prawn, and velvet swimming crab. Molecularly imprinted solid-phase extraction has been evaluated for sample cleanup. The method was characterized in all the matrices in terms of linearity (R 2 ≥ 0.9964), precision (repeatability, RSD ≤ 7.9%, and reproducibility, RSD ≤ 11.1%) and trueness (recoveries between 80.4 and 108.7%). Decision limits, CCα, ranging from 0.2 to 1.5 μg/kg and detection capabilities, CCβ, from 0.2 to 1.8 μg/kg, were obtained.

Keywords

5-Nitroimidazoles Aquaculture products Capillary liquid chromatography Molecularly imprinted solid-phase extraction 

Abbreviations

CCβ

Detection capability

CCα

Decision limit

DMZ

Dimetridazole

EURLs

European Union Reference Laboratories

HMMNI

2-Hydroxymethyl-1-methyl-5-nitro-1H-imidazole

IPZ

Ipronidazole

IPZ-OH

Hydroxyl-ipronidazole

MISPE

Molecularly imprinted solid-phase extraction

MNZ

Metronidazole

MNZ-OH

Hydroxyl-metronidazole

MRLs

Maximum residue levels

5-NDZ

5-Nitroimidazole

ORZ

Ornidazole

RNZ

Ronidazole

SCZ

Secnidazole

SPE

Solid-phase extraction

TNZ

Tinidazole

TRZ

Ternidazole

Notes

Compliance with Ethical Standards

Funding

This study was funded by the Andalusian Government (Junta de Andalucía) for Excellence Project Ref: P12-AGR-1647. M. Hernández-Mesa thanks the “Plan Propio” of University of Granada for a pre-doctoral fellowship. D. Moreno-González acknowledges the program to strengthen research groups of the University of Granada for his postdoctoral contract.

Conflict of Interest

M. Hernández-Mesa declares that he has no conflict of interest. D. Moreno-González declares that he has no conflict of interest. C. Cruces-Blanco declares that she has no conflict of interest. A.M. García-Campaña declares that she has no conflict of interest.

Ethical Approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed Consent

Not applicable.

Supplementary material

12161_2017_928_MOESM1_ESM.docx (454 kb)
ESM 1 (DOCX 453 kb)

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.Department of Analytical Chemistry, Faculty of SciencesUniversity of GranadaGranadaSpain

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