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Determination of Enrofloxacin in Milk Samples Using Silver Nanoparticle Enhanced Terbium-Sensitized Fluorescence Method

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Abstract

Terbium-sensitized fluorescence is one of the simple and sensitive methods for quantification of drugs in biological and food samples. Silver nanoparticles (AgNPs) can enhance the intensity of terbium-sensitized fluorescence and provide a more sensitive method. Enrofloxacin (ENR) is one of the common antibiotics, and the determination of its residue in animal products is recommended because of its problems for human health. Various parameters affecting the fluorescence intensity were optimized using response surface methodology. Then, the optimized parameters were applied to the validation of method for ENR quantification in milk samples. The linearity of the method was from 0.050 to 0.60 mg/L, and the limit of detection (LOD) and limit of quantification (LOQ) of the method were 0.021 to 0.069 mg/L, respectively. The accuracy, precision, and selectivity studies of the developed method give reasonable results. These data showed that the established analysis approach is a simple and sensitive technique for monitoring of ENR in milk samples.

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Acknowledgments

This study was funded by the Vice Chancellor for Research of Tabriz University of Medical Sciences, Tabriz, Iran, for a partial financial support, and it is a part of S. Ershadi’s Pharm.D thesis (No. 3899) registered at Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.

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Authors and Affiliations

  1. Liver and Gastrointestinal Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Saba Ershadi

  2. Faculty of Pharmacy and Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran

    Saba Ershadi

  3. Pharmaceutical Analysis Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran

    Abolghasem Jouyban

  4. Drug Applied Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, 51664, Iran

    Ali Shayanfar

Authors
  1. Saba Ershadi
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  2. Abolghasem Jouyban
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  3. Ali Shayanfar
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Correspondence to Ali Shayanfar.

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Saba Ershadi declares that he has no conflict of interest. Abolghasem Jouyban declares that he has no conflict of interest. Ali Shayanfar declares that he has no conflict of interest.

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Ershadi, S., Jouyban, A. & Shayanfar, A. Determination of Enrofloxacin in Milk Samples Using Silver Nanoparticle Enhanced Terbium-Sensitized Fluorescence Method. Food Anal. Methods 10, 3607–3614 (2017). https://doi.org/10.1007/s12161-017-0914-2

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  • DOI: https://doi.org/10.1007/s12161-017-0914-2

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