Immunity Theory-Based High-Specific Monoclonal Antibody Preparation and Application of Fumonisin B1
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Antibody preparation is the most important step in immunoassays. According to immunity theory (IT) “Antibody tends to recognize the furthest distance of side groups in the coupling league,”; immunogen must be well designed for high-specific antibody production for high-specific antibody preparation. In this research, a novel fumonisin B1 immunogen was synthesized in which protein was conjugated at C2 position to expose the distant two type–feature groups of side chains at C14 and C15 position. After immunization, a high-specific anti-FB1 monoclonal antibody (mAb) was produced with negligible CR value to analogues, including the most structure-related FB2 and FB3. Based on this mAb, a specific ELISA method was developed and validated for FB1 detection in rice with limit of detection at 0.0339 ng kg−1. In real-life samples, 20% were detected positive in 10 samples, with FB1 concentrations at 84 and 20 ng kg−1, respectively.
KeywordsImmunity theory Fumonisin B1 immunogen High-specific monoclonal antibody Immunoassay
This work was financially supported by the National Natural Science Foundation of China (Grant No. 31402246).
Compliance with Ethical Standards
Conflict of Interest
Yanshen Li declares that he has no conflict of interest. Jing Zhang declares that she has no conflict of interest. Yunhui Wang declares that she has no conflict of interest. Xin Mao declares that she has no conflict of interest. Huihui Liu declares that she has no conflict of interest. Chengfeng Sun declares that he has no conflict of interest. Yunguo Liu declares that he has no conflict of interest. Yonglin Gao declares that he has no conflict of interest. Ziping Zhang declares that he has no conflict of interest. Xiulin An declares that she has no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
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