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Quantitative Analysis of Amoxicillin, Amoxicillin Major Metabolites, and Ampicillin in Chicken Tissues via Ultra-Performance Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry

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Abstract

This manuscript describes a rapid, sensitive, and specific approach that utilizes ultra-performance liquid chromatography-electrospray ionization tandem triple quadrupole mass spectrometry (UPLC-ESI/MS/MS) for the selective determination of amoxicillin, amoxicillin metabolites, and ampicillin in chicken tissue samples (muscle, liver, and kidney). This optimized method was validated according to the requirements defined by the European Union and the Food and Drug Administration. The extract obtained after extraction and purification of chicken tissues was injected into a UPLC system coupled to a mass spectrometer operated in the positive electrospray MS/MS mode for analysis. The chicken tissues were spiked with the target compounds at levels of 25.0, 50.0, and 100.0 μg/kg, and the results showed that the extraction recoveries were all higher than 84% and the relative standard deviations did not exceed 20%. The limits of detection and limits of quantification were 0.01–1.36 and 0.05–5.44 μg/kg, respectively. The decision limits were 52.62–57.26 μg/kg, and the detection capabilities were 55.23–64.51 μg/kg. Finally, the new approach was verified through quantitative determination of the analytes in tissues from 30 commercial chickens obtained from local supermarkets.

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Author information

Correspondence to Kaizhou Xie.

Ethics declarations

The study was authorized and undertaken in accordance with the ethics requirements of the official ethical committee of our university.

Funding

This research was financially Supported by the China Agriculture Research System (CARS-42-G23), the National Natural Science Foundation of China (31302009), the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), and the New Century Talent Project of Yangzhou University.

Conflict of Interest

Bo Wang declares that he has no conflict of interest. Maoda Pang declares that he has no conflict of interest. Xing Xie declares that he has no conflict of interest. Min Zhao declares that he has no conflict of interest. Kaizhou Xie declares that he has no conflict of interest. Yangyang Zhang declares that he has no conflict of interest. Xia Zhao declares that he has no conflict of interest. Ya’juan Wang declares that he has no conflict of interest. Ran Wang declares that he has no conflict of interest. Haiqing Wu declares that he has no conflict of interest. Genxi Zhang declares that he has no conflict of interest. Guojun Dai declares that he has no conflict of interest. Jinyu Wang declares that he has no conflict of interest.

Ethical Approval

This article does not present any studies with human participants or animals performed by any of the authors.

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Not applicable.

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Wang, B., Pang, M., Xie, X. et al. Quantitative Analysis of Amoxicillin, Amoxicillin Major Metabolites, and Ampicillin in Chicken Tissues via Ultra-Performance Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry. Food Anal. Methods 10, 3292–3305 (2017). https://doi.org/10.1007/s12161-017-0900-8

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Keywords

  • Amoxicillin
  • Amoxicillin metabolites
  • Ampicillin
  • UPLC-ESI/MS/MS
  • Chicken tissues