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Food Analytical Methods

, Volume 10, Issue 10, pp 3292–3305 | Cite as

Quantitative Analysis of Amoxicillin, Amoxicillin Major Metabolites, and Ampicillin in Chicken Tissues via Ultra-Performance Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry

  • Bo Wang
  • Maoda Pang
  • Xing Xie
  • Min Zhao
  • Kaizhou XieEmail author
  • Yangyang Zhang
  • Xia Zhao
  • Ya’juan Wang
  • Ran Wang
  • Haiqing Wu
  • Genxi Zhang
  • Guojun Dai
  • Jinyu Wang
Article

Abstract

This manuscript describes a rapid, sensitive, and specific approach that utilizes ultra-performance liquid chromatography-electrospray ionization tandem triple quadrupole mass spectrometry (UPLC-ESI/MS/MS) for the selective determination of amoxicillin, amoxicillin metabolites, and ampicillin in chicken tissue samples (muscle, liver, and kidney). This optimized method was validated according to the requirements defined by the European Union and the Food and Drug Administration. The extract obtained after extraction and purification of chicken tissues was injected into a UPLC system coupled to a mass spectrometer operated in the positive electrospray MS/MS mode for analysis. The chicken tissues were spiked with the target compounds at levels of 25.0, 50.0, and 100.0 μg/kg, and the results showed that the extraction recoveries were all higher than 84% and the relative standard deviations did not exceed 20%. The limits of detection and limits of quantification were 0.01–1.36 and 0.05–5.44 μg/kg, respectively. The decision limits were 52.62–57.26 μg/kg, and the detection capabilities were 55.23–64.51 μg/kg. Finally, the new approach was verified through quantitative determination of the analytes in tissues from 30 commercial chickens obtained from local supermarkets.

Graphical Abstract

Keywords

Amoxicillin Amoxicillin metabolites Ampicillin UPLC-ESI/MS/MS Chicken tissues 

Notes

Compliance with Ethical Standards

The study was authorized and undertaken in accordance with the ethics requirements of the official ethical committee of our university.

Funding

This research was financially Supported by the China Agriculture Research System (CARS-42-G23), the National Natural Science Foundation of China (31302009), the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), and the New Century Talent Project of Yangzhou University.

Conflict of Interest

Bo Wang declares that he has no conflict of interest. Maoda Pang declares that he has no conflict of interest. Xing Xie declares that he has no conflict of interest. Min Zhao declares that he has no conflict of interest. Kaizhou Xie declares that he has no conflict of interest. Yangyang Zhang declares that he has no conflict of interest. Xia Zhao declares that he has no conflict of interest. Ya’juan Wang declares that he has no conflict of interest. Ran Wang declares that he has no conflict of interest. Haiqing Wu declares that he has no conflict of interest. Genxi Zhang declares that he has no conflict of interest. Guojun Dai declares that he has no conflict of interest. Jinyu Wang declares that he has no conflict of interest.

Ethical Approval

This article does not present any studies with human participants or animals performed by any of the authors.

Informed Consent

Not applicable.

References

  1. Anfossi P, Zaghini A, Grassigli G, Menotta S, Fedrizzi G (2002) Relative oral bioavailability of microgranulated amoxicillin in pigs. J Vet Pharmacol Ther 25:329–334CrossRefGoogle Scholar
  2. Ang CY, Luo W, Hansen EB Jr, Freeman JP, Thompson HC Jr (1996) Determination of amoxicillin in catfish and salmon tissues by liquid chromatography with precolumn formaldehyde derivatization. J AOAC Int 79:389–396Google Scholar
  3. Becker M, Zittlau E, Petz M (2004) Residue analysis of 15 penicillins and cephalosporins in bovine muscle, kidney and milk by liquid chromatography–tandem mass spectrometry. Anal Chim Acta 520:19–32. doi: 10.1016/j.aca.2004.04.022 CrossRefGoogle Scholar
  4. Bogialli S, Capitolino V, Curini R, Corcia A, Nazzari M, Sergi M (2004) Simple and rapid liquid chromatography-tandem mass spectrometry confirmatory assay for determining amoxicillin and ampicillin in bovine tissues and milk. J Agric Food Chem 52:3286–3291. doi: 10.1021/jf0499572 CrossRefGoogle Scholar
  5. Cass QB, Gomes RF, Calafatti SA, Pedrazolli J (2003) Determination of amoxycillin in human plasma by direct injection and coupled-column high-performance liquid chromatography. J Chromatogr A 987:235–241CrossRefGoogle Scholar
  6. Colin P, de Bock L, T’Jollyn H, Boussery K, van Bocxlaer J (2013) Development and validation of a fast and uniform approach to quantify β-lactam antibiotics in human plasma by solid phase extraction-liquid chromatography-electrospray-tandem mass spectrometry. Talanta 103:285–293. doi: 10.1016/j.talanta.2012.10.046 CrossRefGoogle Scholar
  7. Commission of the European Communities (1999) Commission Regulation (EC) 508/1999 of 4 March 1999 amending Annexes I-IV to Council Regulation (EEC) 2377/90 laying down a community procedure for the establishment of maximum residue limits of veterinary medicinal products in foodstuffs of animal origin. Off J Eur Commun L60:16Google Scholar
  8. Commission of the European Communities (2009) Diario Oficial de las Comunidades Europeas No. 37/2010 L15/1Google Scholar
  9. Dousa M, Hosmanova R (2005) Rapid determination of amoxicillin in premixes by HPLC. J Pharmaceut Biomed 37:373–377. doi: 10.1016/j.jpba.2004.10.010 CrossRefGoogle Scholar
  10. Fagerquist CK, Lightfield AR (2003) Confirmatory analysis of beta-lactam antibiotics in kidney tissue by liquid chromatography/electrospray ionization selective reaction monitoring ion trap tandem mass spectrometry. Rapid Commun Mass Sp 17:660–671. doi: 10.1002/rcm.964 CrossRefGoogle Scholar
  11. Fagerquist CK, Lightfield AR, Lehotay SJ (2005) Confirmatory and quantitative analysis of beta-lactam antibiotics in bovine kidney tissue by dispersive solid-phase extraction and liquid chromatography-tandem mass spectrometry. Anal Chem 77:1473–1482. doi: 10.1021/ac040138q CrossRefGoogle Scholar
  12. Food and Drug Administration (2001) Guidance for industry, bioanalytical method validation. U.S. Department of Health and Human Services, Food and Drug Administration, Center for Drug Evaluation and Research, Center for Veterinary Medicine, Rockville, MDGoogle Scholar
  13. Franski R, Czerniel J, Kowalska M, Franska M (2014) Electrospray ionization collision-induced dissociation tandem mass spectrometry of amoxicillin and ampicillin and their degradation products. Rapid Commun Mass Sp 28:713–722. doi: 10.1002/rcm.6834 CrossRefGoogle Scholar
  14. Freitas A, Barbosa J, Ramos F (2012) Determination of amoxicillin stability in chicken meat by liquid chromatography–tandem mass spectrometry. Food Anal Method 5:471–479CrossRefGoogle Scholar
  15. Gamba V, Dusi G (2003) Liquid chromatography with fluorescence detection of amoxicillin and ampicillin in feeds using pre-column derivatization. Anal Chim Acta 483:69–72. doi: 10.1016/S0003-2670(02)01475-7 CrossRefGoogle Scholar
  16. Hou JP, Poole JW (1971) β-Lactam antibiotics: their physicochemical properties and biological activities in relation to structure. J Pharm Sci 60:503–532. doi: 10.1002/jps.2600600402 CrossRefGoogle Scholar
  17. Ibrahim FA, Nasr JJM (2014) Direct determination of ampicillin and amoxicillin residues in food samples after aqueous SDS extraction by micellar liquid chromatography with UV detection. Anal Method 6:1523–1529. doi: 10.1039/C3AY42011F CrossRefGoogle Scholar
  18. JECFA (Joint FAO/WHO Expert Committee on Food Additives) (2012) Residue evaluation of certain veterinary drugs (seventy-fifth report of the Joint FAO/WHO Expert Committee on Food Additives) FAO JECFA Monographs 12. Food and Agriculture Organization of the United Nations, RomeGoogle Scholar
  19. Liu C, Wang H, Jiang Y, Du Z (2011) Rapid and simultaneous determination of amoxicillin, penicillin G, and their major metabolites in bovine milk by ultra-high-performance liquid chromatography-tandem mass spectrometry. J Chromatogr B 879:533–540. doi: 10.1016/j.jchromb.2011.01.016 CrossRefGoogle Scholar
  20. Menelaou A, Somogyi AA, Barclay ML, Bochner F (1999) Simultaneous quantification of amoxycillin and metronidazole in plasma using high-performance liquid chromatography with photodiode array detection. J Chromatogr B 731:261–266CrossRefGoogle Scholar
  21. Nagele E, Moritz R (2005) Structure elucidation of degradation products of the antibiotic amoxicillin with ion trap MS(n) and accurate mass determination by ESI TOF. J Am Soc Mass Spectr 16:1670–1676. doi: 10.1016/j.jasms.2005.06.002 CrossRefGoogle Scholar
  22. Reyns T, Cherlet M, de Baere S, de Backer P, Croubels S (2008) Rapid method for the quantification of amoxicillin and its major metabolites in pig tissues by liquid chromatography-tandem mass spectrometry with emphasis on stability issues. J Chromatogr B 861:108–116. doi: 10.1016/j.jchromb.2007.11.007 CrossRefGoogle Scholar
  23. Sun L, Jia L, Xie X, Xie K, Wang J, Liu J, Cui L, Zhang G, Dai G (2016) Quantitative analysis of amoxicillin, its major metabolites and ampicillin in eggs by liquid chromatography combined with electrospray ionization tandem mass spectrometry. Food Chem 192:313–318. doi: 10.1016/j.foodchem.2015.07.028 CrossRefGoogle Scholar
  24. Union E (2002) Commission decision of 2002/657/EC implementing council directive 96/23/EC concerning the performance of analytical methods and the interpretation of results (2002/657/EC). Official Journal of European Communities L 221:8–36Google Scholar
  25. US Department of Health and Human Services (2001) Centre for Drug Evaluation and Research, Centre for Veterinary Medicine. http://www.fda.gov/cder/guidance/index.htm.

Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Bo Wang
    • 1
    • 2
    • 3
  • Maoda Pang
    • 4
  • Xing Xie
    • 4
  • Min Zhao
    • 1
    • 2
    • 3
  • Kaizhou Xie
    • 1
    • 2
    • 3
    Email author
  • Yangyang Zhang
    • 1
    • 2
    • 3
  • Xia Zhao
    • 1
    • 2
    • 3
  • Ya’juan Wang
    • 1
    • 2
    • 3
  • Ran Wang
    • 4
  • Haiqing Wu
    • 1
    • 2
    • 3
  • Genxi Zhang
    • 1
    • 2
    • 3
  • Guojun Dai
    • 1
    • 2
    • 3
  • Jinyu Wang
    • 1
    • 2
    • 3
  1. 1.College of Animal Science and TechnologyYangzhou UniversityYangzhouChina
  2. 2.Key Laboratory for Animal Genetics, BreedingReproduction and Molecular Design of Jiangsu ProvinceYangzhouChina
  3. 3.Joint International Research Laboratory of Agriculture & Agri-Product SafetyYangzhou UniversityYangzhouChina
  4. 4.Jiangsu Academy of Agricultural SciencesNanjingChina

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