Quantitative Analysis of Amoxicillin, Amoxicillin Major Metabolites, and Ampicillin in Chicken Tissues via Ultra-Performance Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry
- 356 Downloads
This manuscript describes a rapid, sensitive, and specific approach that utilizes ultra-performance liquid chromatography-electrospray ionization tandem triple quadrupole mass spectrometry (UPLC-ESI/MS/MS) for the selective determination of amoxicillin, amoxicillin metabolites, and ampicillin in chicken tissue samples (muscle, liver, and kidney). This optimized method was validated according to the requirements defined by the European Union and the Food and Drug Administration. The extract obtained after extraction and purification of chicken tissues was injected into a UPLC system coupled to a mass spectrometer operated in the positive electrospray MS/MS mode for analysis. The chicken tissues were spiked with the target compounds at levels of 25.0, 50.0, and 100.0 μg/kg, and the results showed that the extraction recoveries were all higher than 84% and the relative standard deviations did not exceed 20%. The limits of detection and limits of quantification were 0.01–1.36 and 0.05–5.44 μg/kg, respectively. The decision limits were 52.62–57.26 μg/kg, and the detection capabilities were 55.23–64.51 μg/kg. Finally, the new approach was verified through quantitative determination of the analytes in tissues from 30 commercial chickens obtained from local supermarkets.
KeywordsAmoxicillin Amoxicillin metabolites Ampicillin UPLC-ESI/MS/MS Chicken tissues
Compliance with Ethical Standards
The study was authorized and undertaken in accordance with the ethics requirements of the official ethical committee of our university.
This research was financially Supported by the China Agriculture Research System (CARS-42-G23), the National Natural Science Foundation of China (31302009), the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), and the New Century Talent Project of Yangzhou University.
Conflict of Interest
Bo Wang declares that he has no conflict of interest. Maoda Pang declares that he has no conflict of interest. Xing Xie declares that he has no conflict of interest. Min Zhao declares that he has no conflict of interest. Kaizhou Xie declares that he has no conflict of interest. Yangyang Zhang declares that he has no conflict of interest. Xia Zhao declares that he has no conflict of interest. Ya’juan Wang declares that he has no conflict of interest. Ran Wang declares that he has no conflict of interest. Haiqing Wu declares that he has no conflict of interest. Genxi Zhang declares that he has no conflict of interest. Guojun Dai declares that he has no conflict of interest. Jinyu Wang declares that he has no conflict of interest.
This article does not present any studies with human participants or animals performed by any of the authors.
- Ang CY, Luo W, Hansen EB Jr, Freeman JP, Thompson HC Jr (1996) Determination of amoxicillin in catfish and salmon tissues by liquid chromatography with precolumn formaldehyde derivatization. J AOAC Int 79:389–396Google Scholar
- Bogialli S, Capitolino V, Curini R, Corcia A, Nazzari M, Sergi M (2004) Simple and rapid liquid chromatography-tandem mass spectrometry confirmatory assay for determining amoxicillin and ampicillin in bovine tissues and milk. J Agric Food Chem 52:3286–3291. doi: 10.1021/jf0499572 CrossRefGoogle Scholar
- Colin P, de Bock L, T’Jollyn H, Boussery K, van Bocxlaer J (2013) Development and validation of a fast and uniform approach to quantify β-lactam antibiotics in human plasma by solid phase extraction-liquid chromatography-electrospray-tandem mass spectrometry. Talanta 103:285–293. doi: 10.1016/j.talanta.2012.10.046 CrossRefGoogle Scholar
- Commission of the European Communities (1999) Commission Regulation (EC) 508/1999 of 4 March 1999 amending Annexes I-IV to Council Regulation (EEC) 2377/90 laying down a community procedure for the establishment of maximum residue limits of veterinary medicinal products in foodstuffs of animal origin. Off J Eur Commun L60:16Google Scholar
- Commission of the European Communities (2009) Diario Oficial de las Comunidades Europeas No. 37/2010 L15/1Google Scholar
- Food and Drug Administration (2001) Guidance for industry, bioanalytical method validation. U.S. Department of Health and Human Services, Food and Drug Administration, Center for Drug Evaluation and Research, Center for Veterinary Medicine, Rockville, MDGoogle Scholar
- JECFA (Joint FAO/WHO Expert Committee on Food Additives) (2012) Residue evaluation of certain veterinary drugs (seventy-fifth report of the Joint FAO/WHO Expert Committee on Food Additives) FAO JECFA Monographs 12. Food and Agriculture Organization of the United Nations, RomeGoogle Scholar
- Liu C, Wang H, Jiang Y, Du Z (2011) Rapid and simultaneous determination of amoxicillin, penicillin G, and their major metabolites in bovine milk by ultra-high-performance liquid chromatography-tandem mass spectrometry. J Chromatogr B 879:533–540. doi: 10.1016/j.jchromb.2011.01.016 CrossRefGoogle Scholar
- Reyns T, Cherlet M, de Baere S, de Backer P, Croubels S (2008) Rapid method for the quantification of amoxicillin and its major metabolites in pig tissues by liquid chromatography-tandem mass spectrometry with emphasis on stability issues. J Chromatogr B 861:108–116. doi: 10.1016/j.jchromb.2007.11.007 CrossRefGoogle Scholar
- Sun L, Jia L, Xie X, Xie K, Wang J, Liu J, Cui L, Zhang G, Dai G (2016) Quantitative analysis of amoxicillin, its major metabolites and ampicillin in eggs by liquid chromatography combined with electrospray ionization tandem mass spectrometry. Food Chem 192:313–318. doi: 10.1016/j.foodchem.2015.07.028 CrossRefGoogle Scholar
- Union E (2002) Commission decision of 2002/657/EC implementing council directive 96/23/EC concerning the performance of analytical methods and the interpretation of results (2002/657/EC). Official Journal of European Communities L 221:8–36Google Scholar
- US Department of Health and Human Services (2001) Centre for Drug Evaluation and Research, Centre for Veterinary Medicine. http://www.fda.gov/cder/guidance/index.htm.