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Characterization of the Polyphenolic Fingerprint of Origanum × majoricum Cambess. From Argentina by High-Performance Liquid Chromatography and Multivariate Analysis

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Abstract

Origanum × majoricum Cambess. is the most commonly grown oregano in Argentina. The aim of this work was to explore and describe its polyphenolic profile by high-performance liquid chromatography with diode array detection (HPLC-DAD) to support identification by classical microscopy. Remarkable homogeneity was found when defining the HPLC-DAD fingerprint with 17 characteristic common peaks. Eleven spectroscopically identified compounds were located. The method was successfully applied for the characterization of 22 samples from different productive regions of Argentina. The genetic inheritance from both progenitors of this hybrid oregano was evidenced by the presence of compounds coming from Origanum majorana L. and Origanum vulgare L. This method, combined with statistical multivariate analysis, was also useful to recognize two groups within the samples. Although O. × majoricum Cambess. must be considered the only hybrid between two species, two origins considering the subspecies level of O. vulgare L. have been morphologically differentiated for the hybrid, both with O. majorana L. These formulas were differentiated by their HPLC-DAD fingerprints. The Argentine hybrids studied appeared consistently grouped under these two profiles.

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Acknowledgements

This work has been financially supported by the Departamento de Ciencias Básicas of the Universidad Nacional de Luján (Disp No. 427/09). The authors thank Eng. Agr. Otto Brutti, Eng. Agr. Pablo Bauzá, and Dr. Lorena Torres for providing samples of O. × majoricum Cambess. We thank Eng. Agr. Cecilia Xifreda for the pieces of advice and the revision of micrographic observations. We also thank Laboratorio de Control de Calidad Melacrom S.C. for performing the HPLC-DAD chromatograms.

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  1. Basic Sciences Department, Universidad Nacional de Luján, Cruce Av. Carlos Pellegrini y Ruta 7, 6700, Luján, Provincia de Buenos Aires, Argentina

    María Dolores González, Paula Laura Lanzelotti & Cecilia María Luis

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  1. María Dolores González
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  2. Paula Laura Lanzelotti
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  3. Cecilia María Luis
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Correspondence to María Dolores González.

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M. D. González declares that she has no conflict of interest. P. L. Lanzelotti declares that she has no conflict of interest. C. M. Luis declares that she has no conflict of interest.

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González, M.D., Lanzelotti, P.L. & Luis, C.M. Characterization of the Polyphenolic Fingerprint of Origanum × majoricum Cambess. From Argentina by High-Performance Liquid Chromatography and Multivariate Analysis. Food Anal. Methods 10, 3275–3284 (2017). https://doi.org/10.1007/s12161-017-0894-2

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