Food Analytical Methods

, Volume 10, Issue 7, pp 2529–2538 | Cite as

Determination of Natamycin in Dairy Products Using Dispersive Liquid-Liquid Microextraction and Indirect Flame Atomic Absorption Spectrometry

  • Saeed Mohammad Sorouraddin
  • Mir Ali Farajzadeh
  • Abdollah Hassanyani


In this paper, a dispersive liquid-liquid microextraction method has been developed for the extraction and preconcentration of natamycin from cheese and doogh samples and its determination by indirect flame atomic absorption spectrometry. For this purpose, an appropriate mixture of a disperser solvent (ethanol) and an extraction solvent (1,1,2-trichloroethane) is rapidly injected into the samples and Zn2+ cation was added. During this process, natamycin-Zn complex is extracted into fine droplets of the extraction solvent. After centrifugation, the fine droplets of the extractant containing the complex are sedimented at the bottom of a tube with conical bottom. The sedimented phase is injected into the determination system via a home-made sample introduction system. Under the optimal conditions, the linear range was between 5 and 1000 ng mL−1. The limit of detection of the target analyte was obtained 1.8 ng mL−1. The relative recoveries obtained for the spiked cheese and doogh samples were between 86 and 98%. Moreover, the precision of the method was acceptable (<3.9%) in all spiked concentrations. Finally, the method was successfully applied to determine natamycin in cheese and doogh samples.


Natamycin Dispersive liquid-liquid microextraction Flame atomic absorption spectrometry Doogh Cheese 





Dispersive liquid-liquid microextraction


Enrichment factor


Extraction recovery


Flame atomic absorption spectrometry


Hollow fiber-liquid phase microextraction


High performance liquid chromatographic method with diode array detection


Indirect competitive enzyme-linked immunosorbent assay


Linear range


Limit of detection


Liquid chromatography–tandem mass spectrometry


Liquid phase microextraction




Relative standard deviation


Single-drop microextraction

1,1,2,2 –TCE






The authors would like to thank the Research Office at the University of Tabriz for financial support.

Compliance with Ethical Standards


Saeed Mohammad Sorouraddin has received research grants from University of Tabriz.

Conflict of Interest

Saeed Mohammad Sorouraddin declares that he has no conflict of interest. Mir Ali Farajzadeh declares that he has no conflict of interest. Abdollah Hassanyani declares that he has no conflict of interest.

Ethical Approval

This article does not contain any studies with human or animal subjects.

Informed Consent

Not applicable.


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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Saeed Mohammad Sorouraddin
    • 1
  • Mir Ali Farajzadeh
    • 1
  • Abdollah Hassanyani
    • 1
  1. 1.Department of Analytical Chemistry, Faculty of ChemistryUniversity of TabrizTabrizIran

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