Development of an Extraction Method Using Mixture Design for the Evaluation of Migration of Non-target Compounds and Dibutyl Phthalate from Baby Bottles
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This work introduces a simple and rapid method for the extraction of baby bottle migrants from milk simulants employing a mixture of ethyl acetate:dichloromethane:hexane (27.5:22.5:50), using a simplex centroid design for optimization. Initially, the baby bottle materials were identified by FT-IR followed by migration test using 50% EtOH in water at 70 °C/2 h. Next, extraction and identification of migrants were performed by GC-MS. Furthermore, the additives in the baby bottle materials were quantified by HPLC-DAD. On the account of the toxicological potential of dibutyl phthalate, the optimized mixture was used for in-house validation by GC-MS of the proposed method. Dibutyl phthalate (DBP) migration was detected in three baby bottles with a concentration range of 175 to 235 μg kg−1, which is lower than the specific migration limit determined by the Brazilian Health Regulatory Agency. However, exposure to DBP from baby bottles was estimated, and this was higher than the tolerable daily intake recommended by the European Food Safety Authority, indicating a potential public health concern.
KeywordsBaby bottle Polypropylene Phthalate Food contact materials Migrants
This study was carried out with the support of São Paulo Research Foundation (FAPESP), process no. 2014/14248-7 and National Council for Scientific and Technological Development (CNPq) process no. 444909/2014-1.
Compliance with Ethical Standards
Conflict of Interest
Wellington da Silva Oliveira declares no conflict of interest. Thais Cristina Lima de Souza declares no conflict of interest. Marisa Padula declares no conflict of interest. Helena Teixeira Godoy declares no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
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