Food Analytical Methods

, Volume 10, Issue 7, pp 2365–2372 | Cite as

Application of Laser Spectrochemical Analytical Techniques to Follow Up Spoilage of White Meat in Chicken

  • Z. Abdel-Salam
  • S. A. M. Abdel-Salam
  • M. A. Harith


The overall objective of this paper is to evaluate the potential of laser spectrochemical analytical techniques as rapid, cost-effective, and accurate techniques to detect the onset of spoilage in fresh chicken breast fillets in three consecutive days directly following slaughter day. Samples were periodically examined via laser-induced breakdown spectroscopy (LIBS) and laser-induced fluorescence (LIF). In the case of LIBS, the cyanide (CN) and carbon (C2) molecular spectral emission bands in the LIBS spectra of meat have been taken as indicators of protein content in the chicken breast samples. The ratio of ionic to atomic spectral lines of both magnesium and iron is found to be proportional to the chicken meat tenderness which decreases with storage time. LIF has been also exploited as a simple and fast technique for white meat spoilage detection. There was a clear inverse proportionality between the intensity of the samples’ fluorescence band and the storage period. The obtained spectrochemical results have been validated by measuring the total proteins in the investigated samples using a conventional meat analyzer. This work demonstrates the feasibility of adopting LIBS and LIF techniques in characterization of both fresh and spoiled chicken meat samples.


Spectrochemical analysis LIBS LIF White meat Meat spoilage 


Compliance with Ethical Standards

Conflict of Interest

Z. Abdel-Salam declares that she has no conflict of interest. S.A.M. Abdel-Salam declares that he has no conflict of interest. M.A. Harith declares that he has no conflict of interest.

Ethical Approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed Consent

Not applicable.


  1. Abdel-Salam Z, Harith MA (2012) Laser spectrochemical characterization of semen. Talanta 15:140–145CrossRefGoogle Scholar
  2. Abdel-Salam Z, Attala SA, Daoud E, Harith MA (2015) Monitoring of somatic cells in milk via laser analytical techniques for the early detection of mastitis. Dairy Sci Technol 95:331–340CrossRefGoogle Scholar
  3. Abdel-Salam ZA, Abdou AM, Harith MA (2006) Elemental and ultrastructural analysis of the eggshell: Ca, Mg and Na distribution during embryonic development via LIBS and SEM techniques. Int J Poult Sci 5:35–42CrossRefGoogle Scholar
  4. Abdel-Salam Z, Galmed AH, Tognoni E, Harith MA (2007) Estimation of calcified tissues hardness via calcium and magnesium ionic to atomic line intensity ratio in laser induced breakdown spectra. Spectrochimi Acta B 62:1343–1347CrossRefGoogle Scholar
  5. Abdel-Salam Z, Abdel-Ghany S, Harith MA (2014) Evaluation of immunoglobulins in bovine colostrum using laser induced fluorescence. Talanta 129:9–15CrossRefGoogle Scholar
  6. Andersen MB, Frydenvang J, Henckel P, Rinnan A (2016) The potential of laser-induced breakdown spectroscopy for industrial at-line monitoring of calcium content in comminuted poultry meat. Food Control 64:226–223CrossRefGoogle Scholar
  7. Andresen P (2001) Laser induced fluorescence. In: Franz M, Oliver F (eds) Optical measurements techniques and applications, 2nd edn. Springer, Heidelberg New York Dordrecht London, pp 199–228CrossRefGoogle Scholar
  8. Armonk NY (2012). IBM Corp. Released. IBM SPSS statistics for windows, version 21.0.: IBM 484 corpGoogle Scholar
  9. Balamatsia CC, Paleologos EK, Kontominas MG, Savvaidis IN (2006) Correlation between microbial flora, sensory changes and biogenic amines formation in fresh chicken meat stored aerobically or under modified atmosphere packaging at 4 degrees C: possible role of biogenic amines as spoilage indicators. Anton Leeuw Int J G 89:9–17CrossRefGoogle Scholar
  10. Balamatsia CC, Patsias A, Kontominas MG, Savvaidis IN (2007) Possible role of volatile amines as quality-indicating metabolites in modified atmosphere-packaged chicken fillets: correlation with microbiological and sensory attributes. Food Chem 104:1622–1628CrossRefGoogle Scholar
  11. Baudelet M, Boueri M, Yu J, Mao SS, Piscitelli V, Mao X, Russo RE (2007) Time-resolved ultraviolet laser-induced breakdown spectroscopy for organic material analysis. Spectrochim Acta B 62:1329–1334CrossRefGoogle Scholar
  12. By Editor (2016). Detecting adulterated meat with LIBS, 72 spectroscopy 31(2).
  13. Chambers EN, Bowrs JR (1993) Consumer perception of sensory quality in muscle food. Food Technol 47:116–120Google Scholar
  14. Dave D, Ghaly AE (2011) Meat spoilage mechanisms and preservation techniques: a critical review. Am J Agri & Biol Sci 6:486–510CrossRefGoogle Scholar
  15. El-Hussein A, Kassem AK, Ismail H, Harith MA (2010) Exploiting LIBS as a spectrochemical analytical technique in diagnosis of some types of human malignancies. Talanta 82:495–501CrossRefGoogle Scholar
  16. Ellis DI, Broadhurst D, Kell DB, Rowland JJ, Goodacre R (2002) Rapid and quantitative detection of the microbial spoilage of meat by Fourier transform infrared spectroscopy and machine learning. Appl Environ Microbiol 68:2822–2828CrossRefGoogle Scholar
  17. Elnasharty IY, Kassem KA, Sabsabi M, Harith MA (2011) Diagnosis of lubricating oil by evaluating cyanide and carbon molecular emission lines in laser induced breakdown spectra. Spectrochim Acta B 66:588–593CrossRefGoogle Scholar
  18. Fortes FJ, Ctvrtnícková T, Mateo M, Cabalín LM, Nicolas G, Laserna JJ (2012) Spectrochmical study for the in situ detection of oil spill residues using laser induced breakdown spectroscopy. Anal Chim Acta 683:52–57CrossRefGoogle Scholar
  19. Guevara-Franco JA, Alonso-Calleja C, Capita R (2010) Aminopeptidase activity by spoilage bacteria and its relationship to microbial load and sensory attributes of poultry legs during aerobic cold storage. J. Food Protect 73:322–326CrossRefGoogle Scholar
  20. Hassan M, Sighicelli M, Lai A, Colao F, Hanafy AH, Ahmed FR, Harith MA (2008) Studying the enhanced phytoremediation of lead contaminated soils via laser induced breakdown spectroscopy. Spectrochim Acta B 63:1225–1229CrossRefGoogle Scholar
  21. Huis in’t Veld JHJ (1996) Microbial and biochemical spoilage of foods: an overview. International J Food Microbiol 33:1–18CrossRefGoogle Scholar
  22. Jay JM, Loessner MJ, Golden DA (2005) Modern food microbiology, 7th edn. Springer, GermanyGoogle Scholar
  23. Jiménez SM, Salsi MS, Tiburzi MC, Rafaghelli RC, Tessi MA, Coutaz VR (1997) Spoilage microflora in fresh chicken breast stored at 4 °C influence of packaging methods. J Appl Microbiol 83:613–618CrossRefGoogle Scholar
  24. Karlovic S, Ježek D, Marijana Blaži B, Tripalo B, Brnci M, Bosiljkov T, Šimunek M (2009) Influence of refrigeration and ageing time on textural characteristics of fresh meat. Croat J Food Sci Technol 1:1–6Google Scholar
  25. Kasem MA, Russo RE, Harith MA (2011) Influence of biological degradation and environmental effects on the interpretation of archaeological bone samples with laser-induced breakdown spectroscopy. J Anal Atom Spectrom 26:1733–1739CrossRefGoogle Scholar
  26. Lin M, Al-Holy M, Mousavi-Hesary M, Al-Qadiri H, Cavinato AG, Rasco BA (2004) Rapid and quantitative detection of the microbial spoilage in chicken meat by diffuse reflectance spectroscopy (600–1100 nm). Lett Appl Microbiol 39:148–155CrossRefGoogle Scholar
  27. Lucena P, Doña A, Tobaria LM, Laserna JJ (2011) New challenges and insights in the detection and spectral identification of organic explosives by laser induced breakdown spectroscopy. Spectrochim Acta B 66:12–20CrossRefGoogle Scholar
  28. Miller RK (2002) Factors affecting the quality of raw meat. In: Joseph K, John K, Ledward D (eds) Meat processing improving quality. CRC Press, FL, USA, pp 26–63 ISBN: 978-1-59124. PP.484-4Google Scholar
  29. Noll R (2012) Laser-induced breakdown spectroscopy, fundamentals and applications. Springer Verlag, Berlin Heidelberg Dordrecht London New YorkCrossRefGoogle Scholar
  30. Nychas GJE, Skandamis PN, Tassou CC, Koutsoumanis KP (2008) Meat spoilage during distribution. Meat Sci 78:77–89CrossRefGoogle Scholar
  31. Oto N, Oshita S, Makino Y, Kawagoe Y, Sugiyama J, Yoshimura M (2013) Non detractive evaluation of ATP content and plate count on pork meat surface by fluorescence spectroscopy. Meat Sci 93:579–585CrossRefGoogle Scholar
  32. Sahar A, Boubellouta T, DuFour É (2011) Synchronous front-face fluorescence spectroscopy as a promising tool for the rapid determination of spoilage bacteria on chicken breast. Food Res Int 44:471–480CrossRefGoogle Scholar
  33. Sahar A, Dufour É (2014) Use of Fourier transform-infrared spectroscopy to predict spoilage bacteria on aerobically stored chicken breast fillets. LWT-Food Sci Technol 56:315e320CrossRefGoogle Scholar
  34. Soltanizadeh N, Kadivar M, Keramat J, Fazilati M (2008) Comparison of fresh beef and camel meat proteolysis during cold storage. Meat Sci 80:892–889CrossRefGoogle Scholar
  35. Sousa NT, Santos MF, Gomes RC, Brandino HE, Martinez R, Guirro RR (2015) Blue laser inhibits bacterial growth of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. Photomed Laser Surg 33:278–282CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Z. Abdel-Salam
    • 1
  • S. A. M. Abdel-Salam
    • 2
  • M. A. Harith
    • 1
  1. 1.National Institute of Laser Enhanced Science (NILES)Cairo UniversityGizaEgypt
  2. 2.Department of Animal Production, Faculty of AgricultureCairo UniversityGizaEgypt

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