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Establishment of a Lateral Flow Colloidal Gold Immunoassay Strip for the Rapid Detection of Soybean Allergen β-Conglycinin

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Abstract

A rapid lateral flow colloidal gold immunoassay strip was established in a sandwich format with the colloidal gold-labelled mouse anti-β-conglycinin monoclonal antibody (mAb) and the rabbit anti-β-conglycinin polyclonal antibody (pAb) in order to specifically identify a soybean allergen, β-conglycinin. Soybean and soy derivatives have become ubiquitous in many vegetarian and meat-based food products, and as a result, dietary avoidance has become difficult. Therefore, soybean avoidance is challenging for individuals with soybean allergy. In our study, the selected mAb showed high affinity to the target and no cross-reactivity with other soybean allergens. Based on the sandwich immunoassay principle, the rabbit pAb was applied in the test line with high titre. The assay could be accomplished within 10 min without sophisticated procedures. The limit of detection of the test strip was calculated at 1.66 mg/kg in powdered milk using an optical density scanner that measures relative optical density. The assay showed high specificity for β-conglycinin, with no cross-reactions with other food allergens. The efficacy of the lateral flow test strip to detect β-conglycinin in powdered milk samples ranged between 80.8 and 89.2% with relative standard deviations of less than 7.2%. Therefore, the test strip is useful as a rapid and reliable detection method for β-conglycinin in powdered milk. In addition, this assay is suitable for monitoring β-conglycinin in other processed foods when samples are appropriately extracted.

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Authors and Affiliations

  1. College of Food and Bioengineering, Henan University of Science and Technology, Luoyang, Henan, 471023, China

    Yao Wang, Zhaozhou Li, Yafeng Pei & Yuze Hou

  2. Henan Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, 450002, China

    Yao Wang, Qingmei Li, Yaning Sun, Jifei Yang, Yanyan Yang, Yubao Zhi, Ruiguang Deng & Xiaofei Hu

Authors
  1. Yao Wang
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  2. Zhaozhou Li
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  3. Yafeng Pei
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  4. Qingmei Li
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  8. Yubao Zhi
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  9. Ruiguang Deng
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  10. Yuze Hou
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  11. Xiaofei Hu
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Corresponding author

Correspondence to Xiaofei Hu.

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Funding

This research was supported by National Key Technology R&D Program of “12th Five-Year Plan” (2014BAD13B05) and Scientific and Technological Research Project of Henan Province (152102210388) and Basic and Cutting-edge Project of Henan Province (132300413222) and Doctoral Scientific Research Foundation of Henan University of Science and Technology (13480062).

Conflict of Interest

Yao Wang declares that he has no conflict of interest. Zhaozhou Li declares that he has no conflict of interest. Yafeng Pei declares that he has no conflict of interest. Qingmei Li declares that he has no conflict of interest. Yaning Sun declares that he has no conflict of interest. Jifei Yang declares that he has no conflict of interest. Yanyan Yang declares that he has no conflict of interest. Yubao Zhi declares that he has no conflict of interest. Ruiguang Deng declares that he has no conflict of interest. Yuze Hou declares that he has no conflict of interest. Xiaofei Hu declares that he has no conflict of interest.

Ethical Approval

Henan Academy of Agricultural Sciences guidelines for the care and use of animals were followed. This article does not contain any studies with human participants performed by any of the authors.

Informed Consent

Informed consent was obtained from all individual participants included in the study.

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Wang, Y., Li, Z., Pei, Y. et al. Establishment of a Lateral Flow Colloidal Gold Immunoassay Strip for the Rapid Detection of Soybean Allergen β-Conglycinin. Food Anal. Methods 10, 2429–2435 (2017). https://doi.org/10.1007/s12161-017-0800-y

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  • DOI: https://doi.org/10.1007/s12161-017-0800-y

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