Food Analytical Methods

, Volume 10, Issue 7, pp 2281–2292 | Cite as

Development of a New Three-Dimensional Fluorescence Spectroscopy Method Coupling with Multilinear Pattern Recognition to Discriminate the Variety and Grade of Green Tea



According to the different types and contents of amino acids in green tea, a new method was proposed for green tea classification and quality evaluation based on excitation-emission matrix (EEM) fluorescence spectroscopy coupled with multilinear pattern recognition in this work. Amino acids in green tea samples were first derived with formaldehyde and acetyl acetone solution. Derivatives of green teas were then scanned with a three-dimensional fluorescence spectrometry. Multilinear pattern recognition methods, including multilinear principal component analysis (M-PCA), self-weight alternative trilinear decomposition (SWATLD), and multilinear partial least squares discriminant analysis (N-PLS-DA) methods, were used to decompose the EEM data sets. All of these multilinear pattern recognition methods showed the clustering tendency for five different kinds of green tea. Compared with the other two methods, N-PLS-DA got more accurate and reliable classification result because it made full use of all the fluorescence information of the derivative green tea samples. At the same time, this method also revealed the possibility of evaluating the grade of green tea.


Green tea Amino acid Fluorescence derivative Three-dimensional fluorescence spectrometry Multidimensional pattern recognition methods 



The work was financially supported by Foundation of Henan University of Technology (grant no. 2014JCYJ08).

Compliance with Ethical Standards

Conflict of Interest

Leqian Hu declares that he has no conflict of interest. Chunling Yin declares that she has no conflict of interest.

Ethical Approval

This article does not contain any studies with human and animal subjects.

Informed Consent

Not applicable.


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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.School of Chemistry and Chemical EngineeringHenan University of TechnologyZhengzhouChina

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