A New Method Based on Time-Resolved Fluoroimmunoassay for the Detection of Streptomycin in Milk
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Streptomycin (STR), used extensively in the treatment of bovine mastitis, may cause damage such as ototoxicity, allergic reaction, and increasing bacterial resistance to consumers on account of remnant in milk. A time-resolved fluoroimmunoassay (TRFIA) was developed to quantify STR for the first time to ensure food safety. Using secondary antibody labeled with europium (Eu3+) chelate as a tracer, the proposed TRFIA showed that the linear working range was 0.32–5.0 ng/mL under the optimal conditions. Milk samples were deproteinized by trichloroacetic acid and the limit of detection of STR in milk was 1.8 μg/kg. The recoveries of milk samples fortified with 4.0, 20, and 40 μg/kg of STR ranged from 86.2 to 96.3% with relative standard deviations less than 11%. Results of TRFIA for the authentic samples were coincided with those of UHPLC-MS/MS analyses. This study confirmed that the established TRFIA was sensitive as well as reliable and could be an alternative method to monitor STR residue in milk.
KeywordsTime-resolved fluoroimmunoassay Streptomycin Residue Milk
This study was supported financially by grants from the Ministry of Science and Technology of China (2015BAK36B03). The authors would like to thank colleagues at the Department of Veterinary Pharmacology & Toxicology, College of Veterinary Medicine of China Agricultural University, for their technical assistance.
Compliance with Ethical Standards
This study was supported financially by grants from the Ministry of Science and Technology of China (2015BAK36B03).
Conflict of Interest
Yuanze Sun declares that she has no conflict of interest. Jie Xie declares that she has no conflict of interest. Tao Peng declares that he has no conflict of interest. Jianyi Wang declares that he has no conflict of interest. Sanlei Xie declares that he has no conflict of interest. Kai Yao declares that he has no conflict of interest. Cheng Wang declares that he has no conflict of interest. Shujuan Sun declares that she has no conflict of interest. Xi Xia declares that he has no conflict of interest. Haiyang Jiang declares that he has no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
- Aksu H, Cetin O, Arun O, Ergun O (2004) Determination of tetracyclin and streptomycin residues by means of ELISA in pasteurized and UHT-sterilized milk. Med Weter 60:1171–1173Google Scholar
- Arsand JB, Jank L, Martins MT, Hoff RB, Barreto F, Pizzolato TM, Sirtori C (2016) Determination of aminoglycoside residues in milk and muscle based on a simple and fast extraction procedure followed by liquid chromatography coupled to tandem mass spectrometry and time of flight mass spectrometry. Talanta 154:38–45. doi: 10.1016/j.talanta.2016.03.045 CrossRefGoogle Scholar
- Byzova NA, Zvereva EA, Zherdev AV, Eremin SA, Sveshnikov PG, Dzantiev BB (2011) Pretreatment-free immunochromatographic assay for the detection of streptomycin and its application to the control of milk and dairy products. Anal Chim Acta 701:209–217. doi: 10.1016/j.aca.2011.06.001 CrossRefGoogle Scholar
- Commission CA (2014) Maximum residue limits for veterinary drugs in foods. Updated as at the 35th session of the Codex Alimentarius Commission (July 2012) CAC/MRL:2–2012Google Scholar
- European Commission (2002) Commission decision (2002)/657/EC of 12 August (2002) implementing Council Directive 96/23/EC concerning the performance of analytical methods and the interpretation of results. Off J Eur Comm L 221:8–28Google Scholar
- European Commission (2011) Action plan against the rising threats from antimicrobial resistance. http://ec.europa.eu/dgs/health consumer/docs/communication amr 2011 748 en.pdf. Accessed 16 January 2012
- Gremilogianni AM, Megoulas NC, Koupparis MA (2010) Hydrophilic interaction vs ion pair liquid chromatography for the determination of streptomycin and dihydrostreptomycin residues in milk based on mass spectrometric detection. J Chromatogr A 1217:6646–6651. doi: 10.1016/j.chroma.2010.04.059 CrossRefGoogle Scholar
- MOA (2002) No. 235 Bulletin of the Ministry of Agriculture of the People’s Republic of China. Available at http://www.moa.gov.cn/zwllm/tzgg/gg/200302/t20030226_59300.htm Accessed 28.09.12
- NHFPC (2016) National action plan on antimicrobial resistance 2016 to 2020. National Health and Family Planning Commission of China, available at http://www.gov.cn/xinwen/2016-08/25/content_5102348.htm
- Organization WH (2015) Global action plan on antimicrobial resistanceGoogle Scholar
- Regulation C (2010) No. 37/2010 of 22 December 2009 on pharmacologically active substances and their classification regarding maximum residue limits in foodstuffs of animal origin. Off J Eur Union L 15:1–72Google Scholar
- Reverberi R, Reverberi L (2007) Factors affecting the antigen-antibody reaction. Blood Transfus 5:227Google Scholar
- Vinas P, Balsalobre N, Hernandez-Cordoba M (2007) Liquid chromatography on an amide stationary phase with post-column derivatization and fluorimetric detection for the determination of streptomycin and dihydrostreptomycin in foods. Talanta 72:808–812. doi: 10.1016/j.talanta.2006.12.006 CrossRefGoogle Scholar