A New Method Based on Time-Resolved Fluoroimmunoassay for the Detection of Streptomycin in Milk
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Streptomycin (STR), used extensively in the treatment of bovine mastitis, may cause damage such as ototoxicity, allergic reaction, and increasing bacterial resistance to consumers on account of remnant in milk. A time-resolved fluoroimmunoassay (TRFIA) was developed to quantify STR for the first time to ensure food safety. Using secondary antibody labeled with europium (Eu3+) chelate as a tracer, the proposed TRFIA showed that the linear working range was 0.32–5.0 ng/mL under the optimal conditions. Milk samples were deproteinized by trichloroacetic acid and the limit of detection of STR in milk was 1.8 μg/kg. The recoveries of milk samples fortified with 4.0, 20, and 40 μg/kg of STR ranged from 86.2 to 96.3% with relative standard deviations less than 11%. Results of TRFIA for the authentic samples were coincided with those of UHPLC-MS/MS analyses. This study confirmed that the established TRFIA was sensitive as well as reliable and could be an alternative method to monitor STR residue in milk.
KeywordsTime-resolved fluoroimmunoassay Streptomycin Residue Milk
This study was supported financially by grants from the Ministry of Science and Technology of China (2015BAK36B03). The authors would like to thank colleagues at the Department of Veterinary Pharmacology & Toxicology, College of Veterinary Medicine of China Agricultural University, for their technical assistance.
Compliance with Ethical Standards
This study was supported financially by grants from the Ministry of Science and Technology of China (2015BAK36B03).
Conflict of Interest
Yuanze Sun declares that she has no conflict of interest. Jie Xie declares that she has no conflict of interest. Tao Peng declares that he has no conflict of interest. Jianyi Wang declares that he has no conflict of interest. Sanlei Xie declares that he has no conflict of interest. Kai Yao declares that he has no conflict of interest. Cheng Wang declares that he has no conflict of interest. Shujuan Sun declares that she has no conflict of interest. Xi Xia declares that he has no conflict of interest. Haiyang Jiang declares that he has no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
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