Food Analytical Methods

, Volume 10, Issue 7, pp 2175–2184 | Cite as

The Mesoporous Porphyrinic Zirconium Metal-Organic Framework for Pipette-Tip Solid-Phase Extraction of Mercury from Fish Samples Followed by Cold Vapor Atomic Absorption Spectrometric Determination



In this study, the highly stable mesoporous porphyrinic zirconium metal-organic framework, namely PCN-222/MOF-545 (Zr-MOF), was prepared and used for pipette-tip solid-phase extraction of Hg(II). As a high-capacity sorbent, 4 mg of the Zr-MOF was placed into a conventional pipette tip and used, for the first time, for the fast extraction and preconcentration of mercury ions. For desorption, 50 μL of 10% HCl was used by 15 repeated aspirating/dispensing cycles, and Hg ions in elusion were measured by a cold vapor atomic absorption spectrometer. Affecting parameters on extraction efficiency were studied, and optimum conditions were established as amount of sorbent 2 mg, pH was adjusted to 5.0, the eluting volume was 15 μL, and extraction was performed on 1.8 mL of the sample. The optimal number of aspirating/dispensing cycles for extraction and desorption of analytes was found to be 10 and 15 cycles, respectively. The limit of detection of the method was found to be 20 ng L−1 with a relative standard deviation of ≤3.1% (for seven replicate analyses of 20 μg L−1 of mercury). Adsorption capacity and enrichment factor were 35.5 mg g−1 and 120-fold, respectively. The proposed method was successfully applied for the determination of Hg(II) ions in fish samples.


Solid-phase extraction Mesoporous MOF Porphyrin Zirconium metal organic framework (PCN-222/MOF-545) Pipette-tip extraction Fish analysis 


Compliance with Ethical Standards

Conflict of Interest

Mohammad Reza Rezaei Kahkha declares that he has no conflict of interest. Saba Daliran declares that she has no conflict of interest. Ali Reza Oveisi declares that he has no conflict of interest. Massoud Kaykhaii declares that he has no conflict of interest. Zahra Sepehri declares that she has no conflict of interest.

Ethical Approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

Informed Consent

Informed consent is not applicable.


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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.Department of Environmental Health EngineeringZabol University of Medical SciencesZabolIran
  2. 2.Faculty of ChemistryBu-Ali Sina UniversityHamedanIran
  3. 3.Department of Chemistry, Faculty of ScienceUniversity of ZabolZabolIran
  4. 4.Department of Chemistry, Faculty of SciencesUniversity of Sistan and BaluchestanZahedanIran
  5. 5.Department of Internal of MedicineZabol University of Medical SciencesZabolIran

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