Food Analytical Methods

, Volume 10, Issue 7, pp 2346–2357 | Cite as

Determination of Pesticide Residues in Mango Matrices by Ultra High-Performance Liquid Chromatography Coupled with Quadrupole Time-of-Flight Mass Spectrometry

  • P. Sivaperumal
  • A. Salauddin
  • A. Ramesh Kumar
  • K. Santhosh
  • T. Rupal


This study describes an accurate mass measurement of pesticide residues (n = 68) in mango matrices by liquid chromatography coupled with quadrupole time of flight mass spectrometry (UHPLC-Q-TOF/MS). The proposed methodology is based on liquid-liquid extraction with a mixture of 25:75 (v/v) acetonitrile/ethyl acetate followed by dispersive solid phase extraction cleanup (primary secondary amine). Method validation was performed in order to establish accuracy, precision, matrix effect, limit of detection, and limit of quantification. The recoveries at three concentration levels (10, 50, and 100 μg kg−1) were in the range of 70–122% with intra- and inter-day relative standard deviations which were lower than 16%. The detection and quantification limits ranged from 0.5 to 7 μg kg−1 and from 2 to 25 μg kg−1, respectively. The developed method provides good linearity, accuracy, precision, and less matrix effects for the quantitation of selected pesticides. The study confirms that this analytical method can be routinely applied for the analysis of pesticide residues in mango matrices. Finally, the validated method was successfully applied to real samples and no positive samples were found.


Pesticide residue analysis UHPLC-Q-TOF/MS Dual-nebulizer technique Matrix matched calibration Mango matrices 



The authors are grateful to the Director, National Institute of Occupational Health, Ahmedabad, for granting permission to publish the article. The authors acknowledge the Indian Council of Medical Research for the financial assistance of the project (IRIS ID No. 2011-02530).

Compliance with Ethical Standards

Conflict of Interest

P. Sivaperumal declares that he/she has no conflict of interest. A. Salauddin declares that he/she has no conflict of interest. A. Ramesh Kumar declares that he/she has no conflict of interest. K. Santhosh declares that he/she has no conflict of interest. T. Rupal declares that he/she has no conflict of interest.

Ethical Approval

This article does not contain any human or animal matrices.

Informed Consent

Not applicable.

Supplementary material

12161_2016_779_MOESM1_ESM.doc (141 kb)
Table S1 (DOC 141 kb)


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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • P. Sivaperumal
    • 1
  • A. Salauddin
    • 1
  • A. Ramesh Kumar
    • 1
  • K. Santhosh
    • 1
  • T. Rupal
    • 1
  1. 1.National Institute of Occupational HealthIndian Council of Medical ResearchAhmedabadIndia

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