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Novel Two-Dimensional Liquid Chromatography–Tandem Mass Spectrometry for the Analysis of Twenty Antibiotics Residues in Dairy Products

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Abstract

A novel two-dimensional hydrophilic interaction chromatography/reversed phase liquid chromatography–tandem mass spectrometry system was developed to analyze seven categories of antibiotics residues in dairy products. The seven categories were β-lactams, tetracyclines, macrolides, aminoglycosides, amphenicols, quinolones, and sulphonamides, and involved 20 antibiotics. Samples of milk powder or milk samples were extracted by mixed matrix solid phase dispersion using C18 and CN material. The analytes were eluted by acetonitrile and water, and were dried with rotary evaporation. Finally, the residues were dissolved with mobile phase and then analyzed. Under sample pretreatment conditions, chromatographic and mass spectrometric parameters were optimized. Twenty analytes showed good linearities with correlation coefficients of 0.9945 ~ 0.9998. The limits of detection and quantification for milk powder and milk samples were 0.10 ~ 2.40 and 0.33 ~ 7.92 μg/kg, respectively. The proposed method has been applied to the determination of antibiotics residues in milk powder and milk samples.

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Acknowledgements

This study was funded by the China Postdoctoral Science Foundation (2012M521703). We thank Prof. Dongtao Lin of the Sichuan University for copyediting the manuscript.

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Correspondence to Lian Wang.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Lian Wang declares that he has no conflict of interest, Bixia Yang declares that she has no conflict of interest, Xinshen Zhang declares that he has no conflict of interest, and Hongguo Zheng declares that he has no conflict of interest.

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Wang, L., Yang, B., Zhang, X. et al. Novel Two-Dimensional Liquid Chromatography–Tandem Mass Spectrometry for the Analysis of Twenty Antibiotics Residues in Dairy Products. Food Anal. Methods 10, 2001–2010 (2017). https://doi.org/10.1007/s12161-016-0763-4

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