Food Analytical Methods

, Volume 10, Issue 6, pp 2001–2010 | Cite as

Novel Two-Dimensional Liquid Chromatography–Tandem Mass Spectrometry for the Analysis of Twenty Antibiotics Residues in Dairy Products

  • Lian WangEmail author
  • Bixia Yang
  • Xinshen Zhang
  • Hongguo Zheng


A novel two-dimensional hydrophilic interaction chromatography/reversed phase liquid chromatography–tandem mass spectrometry system was developed to analyze seven categories of antibiotics residues in dairy products. The seven categories were β-lactams, tetracyclines, macrolides, aminoglycosides, amphenicols, quinolones, and sulphonamides, and involved 20 antibiotics. Samples of milk powder or milk samples were extracted by mixed matrix solid phase dispersion using C18 and CN material. The analytes were eluted by acetonitrile and water, and were dried with rotary evaporation. Finally, the residues were dissolved with mobile phase and then analyzed. Under sample pretreatment conditions, chromatographic and mass spectrometric parameters were optimized. Twenty analytes showed good linearities with correlation coefficients of 0.9945 ~ 0.9998. The limits of detection and quantification for milk powder and milk samples were 0.10 ~ 2.40 and 0.33 ~ 7.92 μg/kg, respectively. The proposed method has been applied to the determination of antibiotics residues in milk powder and milk samples.


Hydrophilic interaction Two-dimensional liquid chromatography Antibiotics Mixed matrix solid phase dispersion 



This study was funded by the China Postdoctoral Science Foundation (2012M521703). We thank Prof. Dongtao Lin of the Sichuan University for copyediting the manuscript.

Compliance with Ethical Standards

Ethical Approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Conflict of Interest

Lian Wang declares that he has no conflict of interest, Bixia Yang declares that she has no conflict of interest, Xinshen Zhang declares that he has no conflict of interest, and Hongguo Zheng declares that he has no conflict of interest.

Informed Consent

Not applicable.


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Lian Wang
    • 1
    Email author
  • Bixia Yang
    • 2
  • Xinshen Zhang
    • 3
  • Hongguo Zheng
    • 4
  1. 1.Chengdu Centre for Disease Control and PreventionChengduChina
  2. 2.West China School of Public HealthSichuan UniversityChengduChina
  3. 3.National Engineering Laboratory for Clean Technology of Leather ManufactureChengduChina
  4. 4.Thermofisher Scientific China CorporationShanghaiChina

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