Single-Step Multiresidue Determination of β-Lactam Antibiotics and β-Agonists in Porcine Muscle by Liquid Chromatography-Tandem Mass Spectrometry
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A multiresidue method has been optimized and validated for the rapid determination of 12 veterinary drugs belonging to β-agonists and β-lactam antibiotics in porcine muscle. It has been based on liquid chromatography-tandem mass spectrometry and a simple extraction procedure using acetonitrile/H2O. The extract was centrifuged, and the supernatant was directly analyzed by LC-MS/MS in positive ion mode with multiple reaction monitoring (MRM). The linearity of each analyte was almost the coefficient of determination (r) > 0.99. Mean recoveries for all analytes ranged from 74.6 to 115.3% with intra-day RSD ≤ 17.2% and inter-day RSD ≤18.1%. Limits of detection (LODs) and limits of quantification (LOQs) ranged from 0.4 to 2.0 and 1.0 to 8.0 ng/g, respectively. Finally, the validated method was successfully applied to the quantitative analysis of real samples, and clorprenaline was detected in one out of 15 pork samples.
KeywordsMultiresidue analysis Veterinary drug LC-MS/MS Porcine muscle β-Lactam antibiotics β-Agonists
Compliance with Ethical Standards
This work was financially supported by the Science and Technology Project of Henan Province (No. 132102110093).
Conflict of Interest
Juan Li declares that she has no conflict of interest. Yu Chen declares that he has no conflict of interest. Ying-Qian Su declares that she has no conflict of interest. Xiao-Ming Ding declares that she has no conflict of interest. Wen-Shan Xia declares that she has no conflict of interest. Hong-Min Liu declares that he has no conflict of interest. Yan-Bing Zhang declares that she has no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
Informed consent is not applicable for this study.
- Bussche JV, Vanhaecke L, Deceuninck Y, Verheyden K, Wille K, Bekaert K, Le Bizec B, De Brabander HF (2010) Development and validation of an ultra-high performance liquid chromatography tandem mass spectrometry method for quantifying thyreostats in urine without derivatisation. J Chromatogr A 1217:4285–4293CrossRefGoogle Scholar
- Commission Decision 2002/657/EEC (2002) Implementing Council Directive 96/23/EC concerning the performance of analytical methods and the interpretation of results. Off J Eur Commun L221:8–36Google Scholar
- Council Regulation No. 37, 2010 (2010) On pharmacologically active substances and their classification regarding maximum residue limits in foodstuffs of animal origin. Off J Eur Communities L15:1–72Google Scholar
- Wiest L, Bulete A, Giroud B, Fratta C, Amic S, Lambert O, Pouliquen H, Arnaudguilhem C (2011) Multi-residue analysis of 80 environmental contaminants in honeys, honeybees and pollens by one extraction procedure followed by liquid and gas chromatography coupled with mass spectrometric detection. J Chromatogr A 1218:5743–5756CrossRefGoogle Scholar