Food Analytical Methods

, Volume 10, Issue 7, pp 2185–2193 | Cite as

Single-Step Multiresidue Determination of β-Lactam Antibiotics and β-Agonists in Porcine Muscle by Liquid Chromatography-Tandem Mass Spectrometry

Article

Abstract

A multiresidue method has been optimized and validated for the rapid determination of 12 veterinary drugs belonging to β-agonists and β-lactam antibiotics in porcine muscle. It has been based on liquid chromatography-tandem mass spectrometry and a simple extraction procedure using acetonitrile/H2O. The extract was centrifuged, and the supernatant was directly analyzed by LC-MS/MS in positive ion mode with multiple reaction monitoring (MRM). The linearity of each analyte was almost the coefficient of determination (r) > 0.99. Mean recoveries for all analytes ranged from 74.6 to 115.3% with intra-day RSD ≤ 17.2% and inter-day RSD ≤18.1%. Limits of detection (LODs) and limits of quantification (LOQs) ranged from 0.4 to 2.0 and 1.0 to 8.0 ng/g, respectively. Finally, the validated method was successfully applied to the quantitative analysis of real samples, and clorprenaline was detected in one out of 15 pork samples.

Keywords

Multiresidue analysis Veterinary drug LC-MS/MS Porcine muscle β-Lactam antibiotics β-Agonists 

Notes

Compliance with Ethical Standards

Funding

This work was financially supported by the Science and Technology Project of Henan Province (No. 132102110093).

Conflict of Interest

Juan Li declares that she has no conflict of interest. Yu Chen declares that he has no conflict of interest. Ying-Qian Su declares that she has no conflict of interest. Xiao-Ming Ding declares that she has no conflict of interest. Wen-Shan Xia declares that she has no conflict of interest. Hong-Min Liu declares that he has no conflict of interest. Yan-Bing Zhang declares that she has no conflict of interest.

Ethical Approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed Consent

Informed consent is not applicable for this study.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.School of Pharmaceutical Sciences, Institute of Drug Discovery & Development.Zhengzhou UniversityZhengzhouChina
  2. 2.Collaborative Innovation Center of New Drug Research and Safety EvaluationZhengzhouChina

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