A Simple and Rapid Extraction Method to Evaluate the Fatty Acid Composition and Nutritional Value of Goji Berry Lipid
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In this paper, the results of a study on lipid fraction from goji berries are reported. A rapid and simple method based on magnetic stirring with chloroform/methanol mixture and final clean-up with deionized water was developed, which avoided the presence of polar substances in the final extract. The proposed method was compared with conventional (Folch and Soxhlet methods) and unconventional (hexane/2-propanol or methyl-tert-butyl ether extraction) procedures. Sixteen commercial goji samples have been extracted by the developed method; then, the fatty acid composition was determined by high-resolution gas chromatography-flame ionization detector (HRGC-FID) analysis of derivatized samples. Generally, the results obtained underlined the important role of goji berry as a natural source of unsaturated fatty acids (78.0–86.0 %) with a high content of n-6 polyunsaturated fatty acids (PUFA, 48.2–60.2 %), a satisfactory n-6/n-3 PUFA ratio, and a good PUFA/saturated fatty acid ratio.
KeywordsGoji berry Lipid extraction Clean-up HRGC Fatty acid composition
Compliance with Ethical Standards
Conflict of Interest
Francesca Blasi declares that she has no conflict of interest. Domenico Montesano declares that he has no conflict of interest. Maria Stella Simonetti declares that she has no conflict of interest. Lina Cossignani declares that she has no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
- AOAC (1995) Official methods of analysis, 16th edn. AOAC International, ArlingtonGoogle Scholar
- Christie WW (2003) Lipid Analysis, 3rd edn. The Oily Press, BridgwaterGoogle Scholar
- Chung M, Nagella P, Ahn YS, Kim SJ, Ahmad A (2011) Composition of the essential oil and petroleum ether extract of Lycium chinense Miller fruits and antioxidant activity of its several extracts. J Med Plants Res 5:5973–5981Google Scholar
- Endes Z, Uslu N, Özcan MM, Er F (2015) Physico-chemical properties, fatty acid composition and mineral contents of goji berry (Lycium barbarum L.) fruit. J Agroaliment Process Technol 21:36–40Google Scholar
- FAO/WHO (Food and Agriculture Organisation of the United Nations and World Health Organization) (2010) Fats and fatty acids in human nutrition. Report of an expert consultation. FAO Food and Nutrition Paper 91, RomeGoogle Scholar
- Folch JB, Lees M, Sloane Stanley GH (1957) A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226:497–509Google Scholar
- Forino M, Tartaglione L, Dell’Aversano C, Ciminiello P (2016) NMR-based identification of the phenolic profile of fruits of Lycium barbarum (goji berries). Isolation and structural determination of a novel N-feruloyl tyramine dimer as the most abundant antioxidant polyphenol of goji berries. Food Chem 194:1254–1259CrossRefGoogle Scholar
- Guoliang L, Junyou S, Yourui S, Zhiwei S, Lian X, Jie Z, Jinmao Y, Yongjun L (2011) Supercritical CO2 cell breaking extraction of Lycium barbarum seed oil and determination of its chemical composition by HPLC/APCI/MS and antioxidant activity. LWT - Food Sci Technol 44:1172–1178CrossRefGoogle Scholar
- Li G, You J, Suo Y, Song C, Sun Z, Xia L, Zhao H, Shi J (2011) A developed pre-column derivatization method for the determination of free fatty acids in edible oils by reversed-phase HPLC with fluorescence detection and its application to Lycium barbarum seed oil. Food Chem 125:1365–1372CrossRefGoogle Scholar
- Lim TK (ed) (2013) Lycium barbarum. In: Edible medicinal and non-medicinal plants: volume 6, fruits. Springer, London, 240–266Google Scholar
- Montesano D, Cossignani L, Giua L, Urbani E, Simonetti MS, Blasi F (2016) A simple HPLC-ELSD method for sugar analysis in goji berry. J Chem Article ID 6271808. doi: 10.1155/2016/6271808