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Determination of Vanillin and Ethyl-Vanillin in Milk Powder by Headspace Solid-Phase Microextraction Coupled with Gas Chromatography-Mass Spectrometry

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Abstract

A method for determination of vanillin and ethyl-vanillin in milk powder by auto headspace solid-phase microextraction (SPME) coupled with gas chromatography-mass spectrometry (GC-MS) was established. Vanillins in sample were extracted with SPME through a fused-silica fiber coated with divinylbenzene/polydimethylsiloxane. The analytes were desorbed by GC at 260 °C for 5 min and separated on a DB-5MS capillary column and detected by MS with EI source by selected ion monitoring mode (EI-SIM). As a result, the limit of detection of vanillin and ethyl-vanillin was 0.1 and 0.05 mg/kg. The spiked recovery was between 90.0 and 100 %, and the RSD ranged from 1.9 to 5.1 % (n = 6). This method eliminated the interferences from complicated matrix effectively. The method was successfully applied for quick determination of vanillin and ethyl-vanillin content in milk powder samples.

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Funding

This study was funded by the Science and Technology Project of General Administration of Quality Supervision, Inspection and Quarantine of the People’s Republic of China (AQSIQ) (grant nos. 2015IK065 and 2012104020), and the Science and Technology project of Guangdong Entry-Exit Inspection and Quarantine (grant no. 2014GDK62).

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Correspondence to Xiaoqun Wei or Baojun Xu.

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Author Zhiyuan Wang declares he has no conflict of interest. Author Guangfeng Zeng declares that he has no conflict of interest. Author Xiaoqun Wei declares that he has no conflict of interest. Author Bo Ding declares that he has no conflict of interest. Author Cheng Huang declares that he has no conflict of interest. Author Baojun Xu declares that he has no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Wang, Z., Zeng, G., Wei, X. et al. Determination of Vanillin and Ethyl-Vanillin in Milk Powder by Headspace Solid-Phase Microextraction Coupled with Gas Chromatography-Mass Spectrometry. Food Anal. Methods 9, 3360–3366 (2016). https://doi.org/10.1007/s12161-016-0520-8

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  • DOI: https://doi.org/10.1007/s12161-016-0520-8

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