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Identification of Jiaozhou Bay Clams (Ruditapes philippinarum) by Multi-element Fingerprinting Technique

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Abstract

The objective of this study was to develop an analytical method for protecting Jiaozhou Bay clam (Ruditapes philippinarum) with Protected Designation of Origin (PDO). The R. philippinarum samples were collected from three major producing areas (Jiaozhou Bay, Nantong coast, and Dalian coast) in China, and the Meretrix meretrix samples were from Nantong coast in May and September 2014. The contents of 46 elements in all clam samples were determined by inductively coupled plasma mass spectrometry. The obtained data were analyzed using analysis of variance, principal component analysis, and stepwise linear discriminant analysis. The results showed that both the geographical origin and season affected elemental contents in R. philippinarum. Seven elements (Mg, Cd, Sn, Sb, Cs, Ba, and U) independent of seasonal variation were selected as good indicators for discriminating the geographical origin of clams. Based on the discriminant models built with these seven elements, the predictions of the geographic origin gave an overall correct classification rate of 100.0 % and a cross-validation rate of 97.2 %. In addition, R. philippinarum and M. meretrix had their own elemental fingerprinting patterns and could be distinguished. Therefore, the multi-element analysis combined with multivariate statistics can be used for the identification of Jiaozhou Bay clam with PDO.

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Acknowledgements

The authors would like to thank the Institute of Agro-Products Processing Science and Technology, Chinese Academy of Agricultural Sciences, for instrument and technical assistance.

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Authors and Affiliations

  1. Food Science and Engineering College, Qingdao Agricultural University, No. 700, Changcheng Road, Qingdao, 266109, People’s Republic of China

    Haiyan Zhao & Shuangling Zhang

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  1. Haiyan Zhao
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  2. Shuangling Zhang
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Correspondence to Haiyan Zhao.

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Funding

This study was funded by the National Natural Science Foundation of China (No. 31401574) and the Advanced Talents Foundation of Qingdao Agricultural University (No. 1113349).

Conflict of Interest

Haiyan Zhao declares that she has no conflict of interest.

Haiyan Zhao has received research grants from the National Natural Science Foundation of China and the Advanced Talents Foundation of Qingdao Agricultural University.

Shuangling Zhang declares that she has no conflict of interest.

Ethical Approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. This article does not contain any studies with human participants performed by any of the authors.

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Zhao, H., Zhang, S. Identification of Jiaozhou Bay Clams (Ruditapes philippinarum) by Multi-element Fingerprinting Technique. Food Anal. Methods 9, 2691–2699 (2016). https://doi.org/10.1007/s12161-016-0461-2

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  • DOI: https://doi.org/10.1007/s12161-016-0461-2

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