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Food Analytical Methods

, Volume 9, Issue 8, pp 2251–2260 | Cite as

Electrochemical Determination of Vitamin B-12 in Food Samples by Poly(2,2′-(1,4-phenylenedivinylene) Bis-8 hydroxyquinaldine)/Multi-Walled Carbon Nanotube-Modified Glassy Carbon Electrode

  • Hayati FilikEmail author
  • Asiye Aslıhan Avan
  • Sevda Aydar
Article
  • 293 Downloads

Abstract

Poly(2,2′-(1,4-phenylenedivinylene) bis-8-hydroxyquinaldine)/multi-walled carbon nanotube-modified glassy carbon electrode (poly(PBHQ)/MWCNTs/GCE) was developed and applied for the electrochemical estimation of vitamin B-12 (VB-12). Compared to multi-walled carbon nanotube-modified glassy carbon electrode, well-defined redox peaks were observed in phosphate buffer solution at pH 2.5. In contrast with the ill-defined redox peaks observed with unmodified glassy carbon electrode surfaces. The poly(2,2′-(1,4-phenylenedivinylene) bis-8-hydroxyquinaldine-based electrode displayed a good linear range of 0.1 to 10 μM VB-12 with a low detection limit of 0.01 μM. To further study the practical applicability of the proposed sensing procedure, the estimation of real samples was employed with satisfactory consequences. In addition, MWCNTs were used as sorbent for solid phase extraction (SPE) of vitamin B-12 from cereal food samples. Solid phase extraction parameters, such as the amount of MWCNTs, sample volume, pH, and type and amount of the eluent, were optimized.

Keywords

Carbon nanotubes Vitamin B-12 Solid phase extraction Voltammetry Milk analysis Cereal food Pharmaceutical analysis 

Notes

Acknowledgments

We gratefully acknowledge Istanbul University Scientific Research Fund for financial support.

Compliance with Ethical Standard

Conflict of Interest

Asiye Aslıhan Avan and Sevda Aydar declare that they have no conflict of interest.

Ethical Approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed Consent

Human and/or animal were not used in this work.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Hayati Filik
    • 1
    Email author
  • Asiye Aslıhan Avan
    • 1
  • Sevda Aydar
    • 1
  1. 1.Department of Chemistry, Faculty of EngineeringIstanbul UniversityAvcılarTurkey

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