Food Analytical Methods

, Volume 9, Issue 8, pp 2280–2287 | Cite as

Biogenic Amine Content in Red Wines from Different Protected Designations of Origin of Southern Italy: Chemometric Characterization and Classification

  • Raffaella PretiEmail author
  • Giuliana Vinci


Biogenic amines are contaminants naturally present in wines. Their occurrence is influenced by several factors including oenological and agricultural practices, grape variety, and geographical origin. For these reasons, they have been chosen as marker to characterize and classify 56 Italian red wines belonging to four protected designations of origin (PDO) from Southern Italy. Principal component analysis and cluster analysis were applied on data obtained by HPLC/RF in order to highlight the natural grouping of samples. Afterward, linear discriminant analysis and partial least squares were used to classify the wines according to their PDO. Biogenic amines are demonstrated to be a reliable and useful marker for the characterization and classification of the four Southern Italian PDOs investigated. Both the linear discriminant analysis (LDA) and the partial least squares discriminant analysis (PLS-DA) achieved 100 % of wines correctly classified and predicted. Therefore, the determination of these compounds in red wines can play an important role in wine quality assessment, by providing information for the prevention of potential detrimental effects on health and for the characterization of PDO labeled wines.


Red wines Biogenic amines Characterization Multivariate statistical analysis Protected designation of origin 



The authors would like to thank the anonymous reviewers for their helpful comments.

Compliance with Ethical Standards


No funding has been received for this study.

Conflict of Interest

Author Dr. Raffaella Preti declares that she has no conflict of interest. Author Prof. Giuliana Vinci declares that she has no conflict of interest.

Ethical Approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed Consent

Not applicable.


  1. Azcarate SM, Cantarelli MA, Pellerano RG, Marchevsky EJ, Camina JM (2013) Classification of Argentinean Sauvignon blanc wines by UV spectroscopy and chemometric methods. J Food Sci 78:432–436CrossRefGoogle Scholar
  2. Barker M, Rayens W (2003) Partial least squares for discrimination. J Chemometr 17:166–173CrossRefGoogle Scholar
  3. Berrueta LA, Alonso-Salces RM, Heberger K (2007) Supervised pattern recognition in food analysis. J Chrom A 1158:196–214CrossRefGoogle Scholar
  4. Cecchini F, Morassut M (2010) Effect of grape storage time on biogenic amines content in must. Food Chem 123:263–268CrossRefGoogle Scholar
  5. Csomos E, Heberger K, Simon-Sarkadi L (2002) Principal component analysis of biogenic amines and polyphenols in Hungarian wines. J Agric Food Chem 50:3768–3774CrossRefGoogle Scholar
  6. Cynkar W, Dambergs RG, Smith P, Cozzolino D (2010) Classification of Tempranillo wines according to geographic origin: combination of mass spectrometry based electronic nose and chemometrics. Anal Chim Acta 660:227–331CrossRefGoogle Scholar
  7. Dugo G, Vilasi F, La Torre GL, Pellicano TM (2006) Reverse phase HPLC/DAD determination of biogenic amines as dansyl derivatives in experimental red wines. Food Chem 95:672–676CrossRefGoogle Scholar
  8. EC (2009). Commission Regulation (EC) No 607/2009 of 14 July 2009 laying down certain detailed rules for the implementation of Council Regulation (EC) No 479/2008 as regards protected designations of origin and geographical indications, traditional terms, labelling and presentation of certain wine sector products. Official Journal of the European Union, Vol. L 193/60. (pp. 1–80).Google Scholar
  9. EC (2013). European Commission, Agriculture and Rural Development, Agricultural markets, Wine, E-Bacchus. accessed date: Dec 19, 2014. markets/wine/e-bacchus/index.cfm?&language=EN.
  10. Ferreira I, Pinho O (2006) Biogenic amines in Portuguese traditional foods and wines. J Food Prot 69:2293–2303Google Scholar
  11. Forina M, Lanteri S, Armanino C, Cerrato-Oliveiros C, Casolino C (2010) VPARVUS: An extendable package of programs for data explorative analysis, classification and regression analysis. Genova, Italy: Department of Chimica e Tecnologie Farmaceutiche e Alimentari, University of Genova. URL Scholar
  12. Galgano F, Caruso M, Perretti G, Favati F (2011) Authentication of Italian red wines on the basis of the polyphenols and biogenic amines. Eur Food Res Technol 232:889–897CrossRefGoogle Scholar
  13. Guo YY, Yang YP, Peng Q, Han Y (2015) Biogenic amines in wine: a review. Int J Food Sci Technol 50:1523–1532CrossRefGoogle Scholar
  14. Heberger K, Csomos E, Simon-Sarkadi L (2003) Principal component and linear discriminant analyses of free amino acids and biogenic amines in Hungarian wines. J Agric Food Chem 51:8055–8806CrossRefGoogle Scholar
  15. Herbert P, Cabrita MJ, Ratoa N, Laureano O, Alves A (2005) Free amino acids and biogenic amines in wines and musts from the Alentejo region. Evolution of amines during alcoholic fermentation and relationship with variety, sub-region and vintage. J Food Eng 66:315–322CrossRefGoogle Scholar
  16. Landete J, Ferrer S, Polo L, Pardo I (2005) Biogenic amines in wines from three Spanish regions. J Agric Food Chem 53:1119–1124CrossRefGoogle Scholar
  17. Martınez-Pinilla O, Guadalupe Z, Hernandez Z, Ayestaran B (2013) Amino acids and biogenic amines in red varietal wines: the role of grape variety, malolactic fermentation and vintage. Eur Food Res Technol 237:887–895CrossRefGoogle Scholar
  18. Preti R, Antonelli ML, Bernacchia R, Vinci G (2015) Fast determination of biogenic amines in beverages by a core-shell particle column. Food Chem 187:555–562CrossRefGoogle Scholar
  19. Proestos C, Loukatos P, Komaitis M (2008) Determination of biogenic amines in wines by HPLC with precolumn dansylation and fluorimetric detection. Food Chem 106:1218–1224CrossRefGoogle Scholar
  20. Saurina J (2010) Characterization of wines using compositional profiles and chemometrics. Trends Analyt Chem 29:234–245CrossRefGoogle Scholar
  21. Serrano-Lourido D, Saurina J, Hernández-Cassou S, Checa A (2012) Classification and characterisation of Spanish red wines according to their appellation of origin based on chromatographic profiles and chemometric data analysis. Food Chem 135:1425–1431CrossRefGoogle Scholar
  22. Soufleros EH, Bouloumpasi E, Zotou A, Loukou Z (2007) Determination of biogenic amines in Greek wines by HPLC and ultraviolet detection after dansylation and examination of factors affecting their presence and concentration. Food Chem 101:704–716CrossRefGoogle Scholar
  23. Versari A, Laurie VF, Ricci A, Laghi L, Parpinello GP (2014) Progress in authentication, typification and traceability of grapes and wines by chemometric approaches. Food Res Int 60:2–18CrossRefGoogle Scholar
  24. Villamiel M, Polo M, Moreno-Arribas M (2008) Nitrogen compounds and polysaccharides changes during the biological ageing of sherry wines. Food Sci Technol 41:1842–1846Google Scholar
  25. Vinci G, Antonelli ML (2002) Biogenic amines: quality index of freshness in red and white meat. Food Contr 13:519–524CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Department of ManagementLaboratory of Commodity Science, Sapienza University of RomeRomeItaly

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