Food Analytical Methods

, Volume 9, Issue 8, pp 2231–2240 | Cite as

Pesticide Residue Rapid Extraction from Ginseng Tea Using a Modified Luke Method for GC–MS

  • Xuanwei Xu
  • Shuang Liang
  • Yueru LiEmail author
  • Zhongbin LuEmail author


A modified Luke rapid detection method followed by gas chromatography–mass spectrometry (GC–MS) has been developed for the simultaneous determination of 42 pesticides in ginseng tea. This method uses acetone and n-hexane solution to extract and partition pesticides. Ginseng tea samples were mixed with acetone, water and n-hexane solution, and then partitioned by vortex. After the partition, the top layer (n-hexane) was transferred into a centrifuge tube containing primary secondary amine (PSA), graphitized carbon black (GCB) and C18 for purification. And then the centrifuge supernatant was injected into GC–MS. The Luke method was applied in ginseng tea sample detection, and we confirmed that this method can easily extract various types of pesticides from ginseng tea samples. The rates of recovery for pesticides studied were satisfactory, ranging from 65.5 to 109.0 % with a relative standard deviation (RSD) of less than 12 %. The limits of quantification (LOQs) ranged between 0.5 and 11.5 μg·kg−1. The modified Luke method is quick and easy.


Rapid extraction Modified Luke method GC–MS Ginseng tea 


Compliance with Ethical Standards

The manuscript has not been submitted to more than one journal for simultaneous consideration.

The manuscript has not been published previously (partly or in full), unless the new work concerns an expansion of previous work (please provide transparency on the reuse of material to avoid the hint of text recycling).

Consent to submit has been received explicitly from all co-authors, as well as from the responsible authorities—tacitly or explicitly—at the institute/organization where the work has been carried out, before the work is submitted.

Authors whose names appear on the submission have contributed sufficiently to the scientific work and therefore share collective responsibility and accountability for the results.


This study was funded by 2011-Z55 Analysis and Safety Warning System Form of common pesticide residue levels on ginseng in Jilin province.

Conflict of Interest

Xuanwei Xu declares that he has no conflict of interest. Shuang Liang declares that she has no conflict of interest. Yueru Li declares that she has no conflict of interest. Zhongbin Lu declares that he has no conflict of interest.

Ethical Approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed Consent

Informed consent was obtained from all individual participants included in the study.


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Ginseng and Antler Products Testing Center of the Ministry of Agricultural PRCJilin Agricultural UniversityChangchunPeople’s Republic of China
  2. 2.College of Resources and Environment ScienceJilin Agricultural UniversityChangchunPeople’s Republic of China

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