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Food Analytical Methods

, Volume 9, Issue 8, pp 2231–2240 | Cite as

Pesticide Residue Rapid Extraction from Ginseng Tea Using a Modified Luke Method for GC–MS

  • Xuanwei Xu
  • Shuang Liang
  • Yueru LiEmail author
  • Zhongbin LuEmail author
Article

Abstract

A modified Luke rapid detection method followed by gas chromatography–mass spectrometry (GC–MS) has been developed for the simultaneous determination of 42 pesticides in ginseng tea. This method uses acetone and n-hexane solution to extract and partition pesticides. Ginseng tea samples were mixed with acetone, water and n-hexane solution, and then partitioned by vortex. After the partition, the top layer (n-hexane) was transferred into a centrifuge tube containing primary secondary amine (PSA), graphitized carbon black (GCB) and C18 for purification. And then the centrifuge supernatant was injected into GC–MS. The Luke method was applied in ginseng tea sample detection, and we confirmed that this method can easily extract various types of pesticides from ginseng tea samples. The rates of recovery for pesticides studied were satisfactory, ranging from 65.5 to 109.0 % with a relative standard deviation (RSD) of less than 12 %. The limits of quantification (LOQs) ranged between 0.5 and 11.5 μg·kg−1. The modified Luke method is quick and easy.

Keywords

Rapid extraction Modified Luke method GC–MS Ginseng tea 

Notes

Compliance with Ethical Standards

The manuscript has not been submitted to more than one journal for simultaneous consideration.

The manuscript has not been published previously (partly or in full), unless the new work concerns an expansion of previous work (please provide transparency on the reuse of material to avoid the hint of text recycling).

Consent to submit has been received explicitly from all co-authors, as well as from the responsible authorities—tacitly or explicitly—at the institute/organization where the work has been carried out, before the work is submitted.

Authors whose names appear on the submission have contributed sufficiently to the scientific work and therefore share collective responsibility and accountability for the results.

Funding

This study was funded by 2011-Z55 Analysis and Safety Warning System Form of common pesticide residue levels on ginseng in Jilin province.

Conflict of Interest

Xuanwei Xu declares that he has no conflict of interest. Shuang Liang declares that she has no conflict of interest. Yueru Li declares that she has no conflict of interest. Zhongbin Lu declares that he has no conflict of interest.

Ethical Approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed Consent

Informed consent was obtained from all individual participants included in the study.

References

  1. Acosta-Tejada GM, Medina-Peralta S, Moguel-Ordóñez YB, MuñozRodríguez D (2011) Matrix solid-phase dispersion extraction of organophosphorus pesticides from propolis extracts and recovery evaluation by GC/MS. Anal Bioanal Chem 400:885–891CrossRefGoogle Scholar
  2. Andrey S, Igor R, Arkady B, Oleg SB (2013) The use of linear ion trap for qualitative analysis of phytochemicals in Korean ginseng tea. Biomed Chromatogr 27:765–774CrossRefGoogle Scholar
  3. Chen HP, Yin P, Wang QH, Jiang Y, Liu X (2014) A modified QuEChERS sample preparation method for the analysis of 70 pesticide residues in tea using gas chromatography-tandem mass spectrometry. Food Anal Methods 7:1577–1587. doi: 10.1007/s12161-014-9791-0 CrossRefGoogle Scholar
  4. Duda RB, Taback B, Kessel B, Dooley DD, Yang H, Marchiori J, Slomovic BM, Alvarez JG (1996) PS2 expression induced by American ginseng in MCF-7 breast cancer cells. Ann Surg Oncol 3:515–520CrossRefGoogle Scholar
  5. Gamon M, Lleo C, Ten A, Mocholi F (2001) Multiresidue determination of pesticides in fruit and vegetables by gas chromatography–tandem mass spectrometry. J AOAC Int 84(4):1209–1216Google Scholar
  6. Hajslova J, Holadova K, Kocourek V (1998) Matrix-induced effects: a critical point in the gas chromatographic analysis of pesticide residues. J Chromatogr A 800:283–295CrossRefGoogle Scholar
  7. Hiemstra M, Kok AD (2007) Comprehensive multi-residue method for the target analysis of pesticides in crops using liquid chromatography–tandem mass spectrometry. J Chromatogr A 1154:5–21Google Scholar
  8. Khan IA, Allgood J, Walker LA, Abourashed EA, Schlenk D, Benson WH (2001) Determination of heavy metals and pesticides in ginseng products. J AOAC Int 84(3):936–939Google Scholar
  9. Kim HK, Lee KS (2002) Effect of coverings on the growth of ginseng and the persistency of procymidone in growing soils. Korea J Environ Agra 21(1):24–30CrossRefGoogle Scholar
  10. Kim YJ, Jeon JN, Jang MG, Oh JY, Kwon WS, Jung SK, Yang DC (2014) Ginsenoside profiles and related gene expression during foliation in Panax ginseng Meyer. J Ginseng Res 38:66–72CrossRefGoogle Scholar
  11. Lam S, Ng T (2001) Isolation of a small chitinase-like antifungal protein from Panax notoginseng (sanchi ginseng) roots. Int J Biochem Cell B 33:287–292CrossRefGoogle Scholar
  12. Lehotay SJ, Mastovska K, Lightfield AR, Gates RA (2010) Multi-analyst, multi-matrix performance of the QuEChERS approach for pesticide residues in foods and feeds using HPLC/MS/MS analysis with different calibration techniques. J AOAC Int 93:355–367Google Scholar
  13. Li Z, Guo YY, Wu CF, Li X, Wang JH (1999) Protective effects of pseudoginsenoside-F11 on scopolamine-induced memory impairment in mice and rats. J Pharm Pharmacol 51:435–440CrossRefGoogle Scholar
  14. Lu DS, Qiu XL, Feng C, Jin YE, Lin YJ, Xiong LB, Wen YM, Wang DL, Wang GQ (2012) Simultaneous determination of 45 pesticides in fruit and vegetable using an improved QuEChERS method and online gel permeation chromatography–gas chromatography/mass spectrometer. J Chromatogr B 2012(895–896):17–24CrossRefGoogle Scholar
  15. Luke MA, Froberg JE, Masumoto HT (1975) Extraction and cleanup of organochlorine, organophosphate, organonitrogen, and hydrocarbon pesticides in produce for determination by gas-liquid chromatography. J Assoc Off Anal Chem 58:1020–1026Google Scholar
  16.  Luke MA, Froberg JE, Doose GM, Matsumoto HT (1981) Improved multiresidue gas chromatographic determination of organophosphorus, organonitrogen, and organohalogen pesticides in produce, using flame photometric and electrolytic conductivity detectors. J Assoc Off Anal Chem 64:1187–1195Google Scholar
  17. Luo D, Fang B (2008) Structural identification of ginseng polysaccharides and testing of their antioxidant activities. Carbohyd Polym 72:376–381CrossRefGoogle Scholar
  18. Magdalena SB (2015) Validation of a QuEChERS-based gas chromatographic method for multiresidue pesticide analysis in fresh peppermint including studies of matrix effects. Food Anal Methods 8:1413–1424CrossRefGoogle Scholar
  19. Mahady GB, Gyllenhaal C, Fong HHS, Farnsworth NR (2000) Ginseng: a review of safety and efficacy. Nutr Clin Care 3:90–101CrossRefGoogle Scholar
  20. Payá P, Anastassiades M, Mack D, Sigalova I, Tasdelen B, Oliva J, Barba A (2007) Analysis of pesticide residues using the quick easy cheap effective rugged and safe (QuEChERS) pesticide multiresidue method in combination with gas and liquid chromatography and tandem mass spectrometric detection. Anal Bioanal Chem 389:1697–1714CrossRefGoogle Scholar
  21. Pihlström T, Blomkvist G, Friman P, Pagard U, Osterdahl BG (2007) Analysis of pesticide residues in fruit and vegetables with ethyl acetate extraction using gas and liquid chromatography with tandem mass spectrometric detection. Anal Bioanal Chem 389:1773–1789Google Scholar
  22. Psoma AK, Pasias IN, Bletsou AA, Thomaidis NS (2015) Development and validation of a multi-residue method for the determination of pesticides in Chios mastic gum by QuEChERS and liquid chromatography–tandem mass spectrometry. Food Anal Methods 8:624–634CrossRefGoogle Scholar
  23. Pyzunska K (2011) Carbon nanotubes as sorbents in the analysis of pesticides. Chemosphere 83:1407–1413CrossRefGoogle Scholar
  24. Qiu YQ, Lu X, Pang T, Ma CF, Li X, Xu GW (2008) Determination of radix ginseng volatile oils at different ages by comprehensive two-dimensional gas chromatography/time-of-flight mass spectrometry. J Sep Sci 31:3451–3457CrossRefGoogle Scholar
  25. Ravelo-Pérez LM, Hernandez-Borges J, Rodriguez-Delgado MA (2008) Multiwalled carbon nanotubes as efficient solid-phase extraction materials of organophosphorous pesticides from apple, grape, orange and pineapple fruit juices. J Chromatogr A 1211:33–42CrossRefGoogle Scholar
  26. Saieva C, Aprea C, Tumino R, Masala G, Salvini S, Frasca G (2004) Twenty-four-hour urinary excretion of ten pesticide metabolites in healthy adults in two different areas of Italy (Florence and Ragusa). Sci Total Environ 332:71–80CrossRefGoogle Scholar
  27. Shi Y, Sun CJ, Zheng B, Gao B, Sun AM (2013) Simultaneous determination of ten ginsenosides in American ginseng functional foods and ginseng raw plant materials by liquid chromatography tandem mass spectrometry. Food Anal Methods 6:112–122CrossRefGoogle Scholar
  28. Shim JH, Abd El-Aty AM, Choi JH, Choi YS (2007) Post-harvest HPLC determination of chlorfluazuron residues in pears treated with different programs. Biomed Chromatogr 21:695–700CrossRefGoogle Scholar
  29. Steinwandter H (1990) Contributions to the on-line method for the extraction and isolation of pesticide residues and environmental chemicals II. Miniaturization of the on-line method Fresenius Zeitschrift Analytical Chemistry 336:8–11Google Scholar
  30. Steinwandter H (1992) Development of Microextraction methods in residue analysis. In: Cairns T, Sherma J (eds) Emerging strategies for pesticide analysis. CRC, Boca Raton, FL, 3:50 [OpenURL]Google Scholar
  31. Vuksan V, Sievenpiper JL, Koo VYY, Francis T, BeljanZdravkovic U, Xu Z, Vidgen E (2001) American ginseng (Panax quinquefolius L.) reduces postprandial glycemia in nondiabetic subjects and subjects with type 2 diabetes mellitus. Arch Intern Med 160:1009–1013CrossRefGoogle Scholar
  32. Walorczyk S, Gnusowski B (2009) Development and validation of a multi-residue method for the determination of pesticides in honeybees using acetonitrile-based extraction and gas chromatography–tandem quadrupole mass spectrometry. J Chromatogr A 1216:6522–6531CrossRefGoogle Scholar
  33. Wan JY, Fan Y, Yu QT, Ge YZH, Yan CP, Raphael N, Ping L, Ma ZHH, Qi LW (2015) Integrated evaluation of malonyl ginsenosides, amino acids and polysaccharides in fresh and processed ginseng. J Pharm Biomed 107:89–97CrossRefGoogle Scholar
  34. Wong JW, Webster MG, Halverson CA, Hengel MJ, Ngim KK, Ebeler SE (2003) Multiresidue pesticide analysis in wines by solid-phase extraction and capillary gas chromatography-mass spectrometric detection with selective ion monitoring. J Agric Food Chem 51:1148–1461CrossRefGoogle Scholar
  35. Xiang Y, Wang H, Zhang H, Xu PP, Qi XF, Ji Q, Wang XQ (2013) Direct chiral determination of acephate and its metabolite methamidophos in vegetables using QuEChERS by gas chromatography–tandem mass spectrometry. Food Anal Methods 6:133–140CrossRefGoogle Scholar
  36. Zhang Y, Xu H (2014) Determination of triazoles in tea samples using dispersive solid phase extraction combined with dispersive liquid–liquid microextraction followed by liquid chromatography-tandem mass spectrometry. Food Anal Methods 7:189–196CrossRefGoogle Scholar
  37. Zhang JM, Wu YL, Lu YB (2013) Simultaneous determination of carbamate insecticides and mycotoxins in cereals by reversed phase liquid chromatography tandem mass spectrometry using a quick, easy, cheap, effective, rugged and safe extraction procedure. J Chromatogr B 915–916:13–20CrossRefGoogle Scholar
  38. Zhao HX, Zhao SC, Deng LG, Mao JS, Guo CY, Yang GS, Lu X, AboulEnein HY (2013) Rapid determination of organonitrogen, organophosphorus and carbamate pesticides in tea by ultrahighperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Food Anal Methods 6(2):497–505CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Ginseng and Antler Products Testing Center of the Ministry of Agricultural PRCJilin Agricultural UniversityChangchunPeople’s Republic of China
  2. 2.College of Resources and Environment ScienceJilin Agricultural UniversityChangchunPeople’s Republic of China

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