Food Analytical Methods

, Volume 9, Issue 8, pp 2142–2151 | Cite as

Selective Electroanalytical Method for the Determination of Roxarsone in Poultry Feed and Litter

  • Muhammad Waris
  • Jameel Ahmed BaigEmail author
  • Sirajuddin
  • Tasneem Gul Kazi
  • Imam Bakhsh Solangi
  • Samia Siddiqui
  • Hassan Imran Afridi


An extremely sensitive and selective voltammetric method was developed for the determination of roxarsone (ROX) at a newly modified carbon paste microelectrode in Britton–Robinson buffer (BRB) at pH 2.0. The electrode is based on a disposable plastic pipette tip filled with carbon paste based on a mixture of graphite powder and Amberlite XAD-4 (5:1, w/w). The experimental parameters, such as pH, type of the buffer, mode of voltammetry, accumulation potential, accumulation time, and stirring rate were optimized. The reduction peak current dependences were linear for the concentration of ROX from 2.0 to 100 μM. The method showed reproducible results with RSD (n = 11) of <2.0 %. The limit of detection (LOD) and limit of quantification (LOQ) were 0.53 and 1.76 μM, respectively. ROX showed that there was no apparent surface passivation indicating the suitability of the modified electrode for ROX detection. The developed method was successfully applied for the determination of ROX in poultry feed and poultry litter collected from various localities of Hyderabad.


Roxarsone Amberlite XAD-4 resin Carbon paste electrode Poultry feed and litter 



The authors would like to acknowledge the National Centre of Excellence in Analytical Chemistry, University of Sindh, Jamshoro for financial assistance for the completion of current study. Meanwhile, the authors would like to pay thanks to Mr. Oan Muhammad Sahito, Mr. Shahnawaz Baloch, and Mr. Muhammad Kashif Channa for their kind full help during the sampling.

Compliance with Ethical Standards


This study was partial funded by the National Centre of Excellence in Analytical Chemistry, University of Sindh.

Conflict of Interest

Dr. Jameel Ahmed Baig (corresponding author), Mr. Muhammad Waris, Prof. Dr. Sirajuddin, Prof. Dr. Tasneem Gul Kazi, Dr. Hassan Imran Afridi, Dr. Imam Bakhsh Solangi, and Miss Samia Siddiqui declare that they have no conflict of interest.

Ethical Approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed Consent

Not applicable.


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Muhammad Waris
    • 1
  • Jameel Ahmed Baig
    • 1
    Email author
  • Sirajuddin
    • 1
  • Tasneem Gul Kazi
    • 1
  • Imam Bakhsh Solangi
    • 2
  • Samia Siddiqui
    • 1
  • Hassan Imran Afridi
    • 1
  1. 1.Center of Excellence in Analytical ChemistryUniversity of SindhJamshoroPakistan
  2. 2.Dr. M. A. Kazi Institute of ChemistryUniversity of SindhJamshoroPakistan

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