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Food Analytical Methods

, Volume 9, Issue 5, pp 1391–1397 | Cite as

Evaluation of Phytic Acid Content of Some Tea and Nut Products by Reverse-Phase High Performance Liquid Chromatography/Visible Detector

  • Kenan Dost
  • Gülçin Karaca
Article

Abstract

Phytic acid contents of nine different types of nut and six different types of tea and steeped tea were analysed by reverse-phase high performance liquid chromatography with visible detector. The extraction method was based on hydrochloric acid extraction, and the analysis method was based on metal replacement reaction of phytic acid from coloured complex (iron(III)-thiocyanate), separation on CN column and monitoring the absorbance at 460 nm. The retention time for the monitored iron(III)-thiocyanate peak was achieved less than 3 min. The proposed HPLC/Vis procedure shows good linearity over the concentration range of 1–150 mg L−1 with a correlation coefficient value of 0.9938. The effectiveness of metal replacement reaction was presented in terms of relative standard deviation that was 0.62 and 0.88 % for 5 and 50 mg L−1 of phytic acid, respectively. Repeatability of the analytical method was ranging between 1.58–7.88 % (n = 10, for 50 mg L−1) and 0.98–4.63 % (n = 10, for 5 mg L−1) in terms of relative standard deviation. Accuracy of the method is good, ranging relative error between 4.52 and 8.00 % (n = 10, for 50 mg L−1). Phytic acid content is in the range of 1.54 to 9.74 mg g−1 in nuts, 27.67 to 28.82 mg g−1 in green teas, and 20.49 to 21.96 mg g−1 in pocketed roasted teas.

Keywords

Phytic acid HPLC Iron(III)-thiocyanate Iron(III)-phytate Nuts and teas 

Notes

Acknowledgments

The authors are grateful to the Scientific Research Projects Department, Celal Bayar University, for their financial support.

Compliance with Ethical Standards

Funding

This study was funded by the Celal Bayar University Scientific Research Projects Department (grant number BAP 2012-077).

Conflict of Interest

Kenan Dost declares that he has no conflict of interest. Gülçin Karaca declares that she has no conflict of interest.

Ethical Approval

This article does not contain any studies with human participants or animal performed by any of the authors.

Informed Consent

Not applicable.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Department of Chemistry, Faculty of Science and ArtCelal Bayar UniversityManisaTurkey

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