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Multiplex Immunogold Chromatographic Assay for Simultaneous Determination of Macrolide Antibiotics in Raw Milk

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A rapid, simple, reliable, and sensitive immunogold chromatographic assay (IGCA) was described for simultaneous determination of four macrolide antibiotics (erythromycin, spiramycin, tilmicosin, and tylosin) in raw milk. Three antigens were immobilized as three test lines on the nitrocellulose membrane, which enable the simultaneous determination on a single test strip. Samples were detected directly without treatment; the entire testing process was completed within 10 min. The visual detection limits for erythromycin, spiramycin, tilmicosin, and tylosin were 5, 5, 10, and 20 ng/mL, respectively. Sixty blind raw milk samples were analyzed by both IGCA and liquid chromatography–tandem mass spectrometry; the results showed a good correlation between the two methods. The results demonstrate that the developed method could provide a rapid and effective approach for the onsite determination of multi-macrolide antibiotics residues in a large number of samples.

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This work is supported financially by an National Key Technology R&D Program from the Ministry of Science and Technology (No. 2012BAK17B16), and the Trans-Century Training Programme Foundation for Talents by the Ministry of Education (NCET-12–0529) and the State Key Program of the National Natural Science of China (No. 30830082).

Conflict of Interest

Xiangmei Li has no competing financial interests. Kai Wen has no competing financial interests. Yiqiang Chen has no competing financial interests. Xiaoping Wu has no competing financial interests. Xingyao Pei has no competing financial interests. Qi Wang has no competing financial interests. Ailing Liu has no competing financial interests. Jianzhong Shen has no competing financial interests. Guidelines for the care and use of laboratory animals were followed.

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Correspondence to Jianzhong Shen.

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Li, X., Wen, K., Chen, Y. et al. Multiplex Immunogold Chromatographic Assay for Simultaneous Determination of Macrolide Antibiotics in Raw Milk. Food Anal. Methods 8, 2368–2375 (2015).

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