Abstract
Fatty acids (FAs) play many essential roles in biological systems, and they are the aim of different research studies due to their benefits on human health. Milk and dairy products contribute significantly to the consumption of FAs in the human diet. In consequence, manipulation of FA composition of cows’ milk via nutritional strategies has been an important target for the dairy industry and a challenge from an analytical point of view. Milk FA composition is complex, and their analysis involves multiple steps (extraction procedure, methylation, FA methyl ester extraction and gas chromatography (GC) determination) that turn it into a tedious and time-consuming procedure. In recent years, some efforts have been made to develop an analytical approach with simultaneous extraction and derivatization of FAs. In this sense, microwave (MW)-assisted digestion and extraction methods have been used for many years and today can be considered standard operating procedures in many laboratories. It is a powerful tool for different analytical methodology development. This study is focused on one-step extraction/transmethylation MW-assisted methodology feasibility for FA analysis in milk compared to a reference method.
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Acknowledgments
The authors wish to thank the laboratory staff of the Department of Animal Nutrition Grassland and Forages at the Regional Institute of Agro-food, Research and Development (SERIDA) for their assistance, especially to Alejandro Argamentería and Alfonso Carballal, for their invaluable help in the statistical analysis of the experimental results. This study was financially supported by the Spanish Project RTA2012-00063-C02-00 from the INIA and PC10-54 from the Asturias Regional Project and European Regional Development Fund (ERDF). The authors also want to thank the Spanish Reference Laboratory for milk and milk products (EA Search Facility, no. 517/LE1040) for providing butter reference material.
Compliance with Ethics Requirements
This article does not contain any studies with human or animal subjects
Conflict of Interest
The authors have no financial relationship with the organization that sponsored the research. All authors declare that they have no conflict of interest.
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González-Arrojo, A., Soldado, A., Vicente, F. et al. Microwave-Assisted Methodology Feasibility for One-Step Extraction and Transmethylation of Fatty Acids in Milk for GC-Mass Spectrometry. Food Anal. Methods 8, 2250–2260 (2015). https://doi.org/10.1007/s12161-015-0108-8
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DOI: https://doi.org/10.1007/s12161-015-0108-8