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Development of a New Certified Reference Material of Kaempferol by Using Differential Scanning Calorimetry, Coulometric Titration Method, and Mass Balance Method

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Abstract

Dietary flavonols have been found to possess preventive and therapeutic potential against several kinds of cancers. According to epidemiologic investigation data, kaempferol is a major component of flavonols present in various natural sources such as herbs, vegetables, and fruits. The purity of kaempferol in candidate certified reference material (CRM) was based on the agreement of results obtained by three different methods, including differential scanning calorimetry (DSC), coulometric titration (CT), and mass balance method. The certified value of kaempferol CRM is 99.5 % with an expanded uncertainty of 0.2 % (k = 2). The new CRM of kaempferol can be used as a valuable tool to validate measurement methods, to evaluate analytical results, and to control the quality of kaempferol-related traditional herbs, foods, dietary supplements, and pharmaceutical formulations.

Development of a New Certified Reference Material of Kaempferol

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Acknowledgments

This work was supported by the National Twelfth Five Year Science and Technology Support Program (2012ZX09301002-001-013), the Research Special Fund for Public Welfare Industry of Health (200902008), and the basic research program of Institute of Materia Medica (2013CHX20) are gratefully acknowledged.

Compliance with Ethics Requirements

This article does not contain any studies with human or animal subjects.

Conflict of Interest

Ningbo Gong declares that he has no conflict of interest. Guoshun Zhang declares that he has no conflict of interest. Baoxi Zhang declares that he has no conflict of interest. Guanhua Du declares that he has no conflict of interest. Zhaolin Gao declares that he has no conflict of interest. Yang Lu declares that he has no conflict of interest.

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Authors and Affiliations

  1. Beijing Key Laboratory of Polymorphic Drugs, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100050, China

    Ningbo Gong, Guoshun Zhang, Baoxi Zhang & Yang Lu

  2. Beijing City Key Laboratory of Drug Target Identification and Drug Screening, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100050, China

    Guanhua Du

  3. Key Laboratory of Polymorph Pharmaceutical of Shandong Province, Tengzhou, 277500, China

    Zhaolin Gao & Yang Lu

Authors
  1. Ningbo Gong
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  2. Guoshun Zhang
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  3. Baoxi Zhang
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  4. Guanhua Du
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  5. Zhaolin Gao
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  6. Yang Lu
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Corresponding authors

Correspondence to Guanhua Du or Yang Lu.

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Gong, N., Zhang, G., Zhang, B. et al. Development of a New Certified Reference Material of Kaempferol by Using Differential Scanning Calorimetry, Coulometric Titration Method, and Mass Balance Method. Food Anal. Methods 8, 2347–2355 (2015). https://doi.org/10.1007/s12161-015-0098-6

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  • DOI: https://doi.org/10.1007/s12161-015-0098-6

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