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High-Sensitive Chemiluminescent ELISA Method Investigation for the Determination of Deoxynivalenol in Rice

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Abstract

In this research, a reliable and rapid chemiluminescent ELISA (CL-ELISA) determination procedure was developed and validated for the detection of deoxynivalenol (DON) in rice. With the help of chemiluminescent substrate, this developed protocol exhibited a high-sensitive character with a limit of detection (LOD) value of 0.94 ng/mL while it was 6.12 ng/mL in our previous report with the conventional colorimetric ELISA procedure by using the same monoclonal antibody (mAb). The mAb used in this research was proved to tolerate no more than 20 % methanol. Therefore, after the extraction procedure with 20 % methanol, samples could be proceeded to take the detection steps directly without dilution by PBS. In spiked rice samples, mean recoveries ranged from 91.40 to 93.48 % with intra-day and inter-day relative standard deviation (RSD) less than 10.62 and 12.41 %, respectively. The results demonstrated that this developed high-sensitive CL-ELISA immunoassay was suitable for screening of DON in rice samples.

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Acknowledgments

This work was supported by Development Project of Shandong Province Science and Technology (no. 2013YD15011).

Compliance with Ethics Requirements

Yanshen Li declares that he has no competing financial interests. Gongzhen Liu declares that he has no competing financial interests. Xuejun Fu declares that he has no competing financial interests. Jun He declares that he has no competing financial interests. Zhanhui Wang declares that he has no competing financial interests. Jianhai Hou declares that he has no competing financial interests. Xingyuan Cao declares that he has no competing financial interests. Weimin Shi declares that she has no competing financial interests. Suxia Zhang declares that she has no competing financial interests. This article does not contain any studies with human or animal subjects.

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Authors and Affiliations

  1. College of Life Science, Yantai University, Yantai, Shandong, 264005, People’s Republic of China

    Yanshen Li, Xuejun Fu, Jun He & Jianhai Hou

  2. Shandong Institute of Parasitic Diseases, Jining, Shandong, 272033, People’s Republic of China

    Gongzhen Liu

  3. Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing, 100193, People’s Republic of China

    Zhanhui Wang, Xingyuan Cao, Weimin Shi & Suxia Zhang

Authors
  1. Yanshen Li
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  2. Gongzhen Liu
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  3. Xuejun Fu
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  4. Jun He
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  5. Zhanhui Wang
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  6. Jianhai Hou
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  7. Xingyuan Cao
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  8. Weimin Shi
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  9. Suxia Zhang
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Corresponding authors

Correspondence to Yanshen Li or Suxia Zhang.

Additional information

Yanshen Li and Gongzhen Liu contributed equally to this study.

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Li, Y., Liu, G., Fu, X. et al. High-Sensitive Chemiluminescent ELISA Method Investigation for the Determination of Deoxynivalenol in Rice. Food Anal. Methods 8, 656–660 (2015). https://doi.org/10.1007/s12161-014-9941-4

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  • DOI: https://doi.org/10.1007/s12161-014-9941-4

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