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Drop-Type Chemiluminescence (DCL) System and Sample Treatment Platform Using Magnetic Nanoparticles to Determine Enrofloxacin and Its Metabolite in a Chicken Meat

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Abstract

An analytical method to determine antibiotics using a lab-built drop-type chemiluminescence (DCL) system is introduced. Magnetic nanoparticles (MNPs) functionalized with amine groups were used for the extraction of antibiotics from a pretreated sample solution by magnetic separation. Detection is achieved with an antibody specific against enrofloxacin and ciprofloxacin and a secondary horseradish peroxidase (HRP)-labelled antibody. The enzyme is capable of catalyzing the luminol reaction, which can be read out with the DCL device. For application, the DCL system was used to determine enrofloxacin and its metabolite in chicken meat. The obtained detection limits were 0.039 pmol/mL (~0.12 ng/mL) and 0.022 pmol/mL for enrofloxacin and ciprofloxacin, respectively, which are about 10 times better than enzyme-linked immunosorbent assay (ELISA).

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Acknowledgments

This work was supported by the Korea Food and Drug Administration in 2012 (10162KFDA995).

Conflict of Interest

H. B. Lim declares that he has no conflict of interest. Jungwon Ahn declares that she has no conflict of interest.

Compliance with Ethics Requirements

For demonstration of the developed method in this work, chicken meat treated for food was purchased from local food market. All institutional and national guidelines for the care and use of laboratory animals were followed.

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Authors and Affiliations

  1. Department of Chemistry, NSBI, Dankook University, 126 Jukjeon-dong, Suji-gu, Yongin-si, Gyeonggi-do, 448-701, South Korea

    Jungwon Ahn & H. B. Lim

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  1. Jungwon Ahn
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  2. H. B. Lim
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Correspondence to H. B. Lim.

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Ahn, J., Lim, H.B. Drop-Type Chemiluminescence (DCL) System and Sample Treatment Platform Using Magnetic Nanoparticles to Determine Enrofloxacin and Its Metabolite in a Chicken Meat. Food Anal. Methods 8, 79–85 (2015). https://doi.org/10.1007/s12161-014-9871-1

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  • DOI: https://doi.org/10.1007/s12161-014-9871-1

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