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Simultaneous Determination of 16 Organic Acids in Food by Online Enrichment Ion Chromatography−Mass Spectrometry

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Abstract

A novel analytical method for simultaneous determination of 16 organic acids in foodstuff using online enrichment ion chromatography−mass spectrometry (IC–MS) was developed. The online enrichment and separation of the organic acids were performed using ion chromatography with a homemade enrichment column and a homemade separation column. The qualitative and quantitative analyses of the organic acids were performed using mass spectrometry in the selected ion monitoring (SIM) mode and atmospheric pressure chemical ionization (APCI) source in a negative mode. Two hundred-microliter samples were injected for analysis using an online enrichment time of 3 min. Sodium hydroxide solution (NaOH) was used for the gradient elution system. The two columns made it possible to have a low limit of detection since it has good enriching and separating capabilities. Sixteen kinds of organic acids were separated completely within 30 min. All the curves showed good linearity (R 2 ≥ 0.9949) within the test ranges. The limits of detection (LODs) were between 0.01 and 0.22 mg/L, and the average recoveries were between 80.5 and 109.8 %. The relative standard deviations (RSDs) were less than 8.02 %. The results indicated that this method was simple, rapid, sensitive, and accurate for the determination of organic acids in various food products.

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Compliance with Ethics Requirements

No conflict of interest exists in the submission of this manuscript, and manuscript is approved by all authors for publication. This work described was an original research that has not been published previously and not under consideration for publication elsewhere, in whole or in part. All the authors listed have approved the manuscript that is enclosed.

Zhiyu Xiong has no conflict of interest. Ying Dong has no conflict of interest. Hongbin Zhou has no conflict of interest. HuiWang has no conflict of interest. Yunxia Zhao has no conflict of interest. This article does not contain any studies with human or animal subjects.

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Authors and Affiliations

  1. School of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013, Jiangsu Province, People’s Republic of China

    Zhiyu Xiong & Ying Dong

  2. State Key Laboratory of Food Additive and Condiment Testing, Zhenjiang Entry-Exit Inspection and Quarantine Bureau, Zhenjiang, 212008, Jiangsu Province, People’s Republic of China

    Hongbin Zhou, Hui Wang & Yunxia Zhao

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  1. Zhiyu Xiong
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  2. Ying Dong
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  3. Hongbin Zhou
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  4. Hui Wang
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  5. Yunxia Zhao
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Correspondence to Ying Dong or Hongbin Zhou.

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Xiong, Z., Dong, Y., Zhou, H. et al. Simultaneous Determination of 16 Organic Acids in Food by Online Enrichment Ion Chromatography−Mass Spectrometry. Food Anal. Methods 7, 1908–1916 (2014). https://doi.org/10.1007/s12161-014-9839-1

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  • DOI: https://doi.org/10.1007/s12161-014-9839-1

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