Skip to main content

Advertisement

SpringerLink
Log in

Development and Optimization of a SERS Method for On-site Determination of Nitrite in Foods and Water

  • Published:
Food Analytical Methods Aims and scope Submit manuscript

Abstract

A rapid, sensitive, and selective detection method of nitrite in foods and water was established by surface-enhanced Raman spectroscopy (SERS) based on the diazo reaction of nitrite with p-nitroaniline and 1-naphthylamine in acidic solution. The azo dye (4-(4-nitrophenyldiazenyl)naphthalene-1-aminium, NNA), which was derived from the diazo reaction, was determined by SERS using citrate-coated silver nanoparticles (AgNPs) as SERS substrates. The concentrations of nitrite in samples were finally calculated from the intensities of SERS signals generated by NNA in the testing solutions. By using the present method combined with a portable miniature Raman spectrometer, on-site determination of nitrite could be performed easily and efficiently. The effects of several experimental parameters on the intensity of SERS signals, such as the volume of AgNP solution and the mixing time of AgNPs and NNA, were investigated. The linear range of the method was 0.1–10.0 mg L−1. The limits of detection (LODs) were 0.01, 0.03, and 0.05 mg L−1 at 720, 1,459, and 1,609 cm−1, respectively. The method was applied to the determination of nitrite in real food and water samples, with recoveries in the range of 86.9–103.4 % and relative standard deviations (RSDs) less than 9.64 %.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

Similar content being viewed by others

References

  • Cabaleiro N, De La Calle I, Gil S, Pena FJ, Costas M, Bendicho C, Lavilla I (2010) Simultaneous ultrasound-assisted emulsification-derivatization as a simple and miniaturized sample preparation method for determination of nitrite in cosmetic samples by microvolume UV–Vis spectrophotometry. Talanta 83(2):386–390

    Article  CAS  Google Scholar 

  • Chen Q, Ai S, Fan H, Cai J, Ma Q, Zhu X, Yin H (2010) The immobilization of cytochrome c on MWNT-PAMAM-Chit nanocomposite incorporated with DNA biocomposite film modified glassy carbon electrode for the determination of nitrite. J Solid State Electr 14(9):1681–1688

    Article  CAS  Google Scholar 

  • Chen Y, Wu L, Chen Y, Bi N, Zheng X, Qi H, Qin M, Liao X, Zhang H, Tian Y (2012) Determination of mercury(II) by surface-enhanced Raman scattering spectroscopy based on thiol-functionalized silver nanoparticles. Microchim Acta 177(3–4):341–348

    Article  CAS  Google Scholar 

  • Di Anibal CV, Marsal LF, Callao MP, Ruisanchez I (2012) Surface Enhanced Raman Spectroscopy (SERS) and multivariate analysis as a screening tool for detecting Sudan I dye in culinary spices. Spectrochim Acta A 87:135–141

    Article  Google Scholar 

  • Dunham AJ, Barkley RM, Sievers RE (1995) Aqueous nitrite ion determination by selective reduction and gas phase nitric oxide chemiluminescence. Anal Chem 67(1):220–224

    Article  CAS  Google Scholar 

  • Efrima S, Metiu H (1979) Light scattering by a molecule near a solid surface. II. Model calculations. J Chem Phys 70:2297

    Article  CAS  Google Scholar 

  • Faulds K, Barbagallo RP, Keer JT, Smith WE, Graham D (2004) SERRS as a more sensitive technique for the detection of labelled oligonucleotides compared to fluorescence. Analyst 129(7):567–568

    Article  CAS  Google Scholar 

  • Gopalan AI, Lee KP, Komathi S (2010) Bioelectrocatalytic determination of nitrite ions based on polyaniline grafted nanodiamond. Biosens Bioelectron 26(4):1638–1643

    Article  CAS  Google Scholar 

  • Goulet PJG, Aroca RF (2007) Distinguishing individual vibrational fingerprints: single-molecule surface-enhanced resonance Raman scattering from one-to-one binary mixtures in Langmuir–Blodgett monolayers. Anal Chem 79(7):2728–2734

    Article  CAS  Google Scholar 

  • Irandoust M, Shariati-Rad M, Haghighi M (2013) Nitrite determination in water samples based on the modified Griess reaction and central composite design. Anal Methods 5(20):5977–5982

    Article  CAS  Google Scholar 

  • Ito K, Nomura R, Fujii T, Tanaka M, Tsumura T, Shibata H, Hirokawa T (2012) Determination of nitrite, nitrate, bromide, and iodide in seawater by ion chromatography with UV detection using dilauryldimethylammonium-coated monolithic ODS columns and sodium chloride as an eluent. Anal Bioanal Chem 404(8):2513–2517

    Article  CAS  Google Scholar 

  • Jin Y, Ma P, Liang F, Gao D, Wang X (2013) Determination of malachite green in environmental water using cloud point extraction coupled with surface-enhanced Raman scattering. Anal Methods 5(20):5609–5614

    Article  CAS  Google Scholar 

  • Kiefer W, Schlücker S (2011) Surface enhanced Raman spectroscopy: analytical, biophysical and life science applications. Wiley, Chichester

    Google Scholar 

  • Kim K, Kim KL, Shin KS (2012) Selective detection of aqueous nitrite ions by surface-enhanced Raman scattering of 4-aminobenzenethiol on Au. Analyst 137(16):3836–3840

    Article  CAS  Google Scholar 

  • Kunov-Kruse AJ, Kristensen SB, Liu C, Berg RW (2011) Experimental and ab initio DFT calculated Raman spectrum of Sudan I, a red dye. J Raman Spectrosc 42(6):1470–1478

    Article  CAS  Google Scholar 

  • Lee PC, Meisel D (1982) Adsorption and surface-enhanced Raman of dyes on silver and gold sols. J Phys Chem 86(17):3391–3395

    Article  CAS  Google Scholar 

  • Li D, Qu L, Zhai W, Xue J, Fossey JS, Long Y (2011) Facile on-site detection of substituted aromatic pollutants in water using thin layer chromatography combined with surface-enhanced Raman spectroscopy. Environ Sci Technol 45(9):4046–4052

    Article  CAS  Google Scholar 

  • Lin M, He L, Awika J, Yang L, Ledoux DR, Li H, Mustapha A (2008) Detection of melamine in gluten, chicken feed, and processed foods using surface enhanced Raman spectroscopy and HPLC. J Food Sci 73(8):T129–T134

    Article  CAS  Google Scholar 

  • Liu B, Han G, Zhang Z, Liu R, Jiang C, Wang S, Han M-Y (2012) Shell thickness-dependent Raman enhancement for rapid identification and detection of pesticide residues at fruit peels. Anal Chem 84(1):255–261

    Article  CAS  Google Scholar 

  • Lombardi JR, Birke RL, Lu T, Xu J (1986) Charge-transfer theory of surface enhanced Raman spectroscopy: Herzberg–Teller contributions. J Chem Phys 84:4174

    Article  CAS  Google Scholar 

  • Ma P, Liang F, Sun Y, Jin Y, Chen Y, Wang X, Zhang H, Gao D, Song D (2013) Rapid determination of melamine in milk and milk powder by surface-enhanced Raman spectroscopy and using cyclodextrin-decorated silver nanoparticles. Microchim Acta 180(11–12):1173–1180

    Article  CAS  Google Scholar 

  • Marques Luiz VH, Pezza L, Pezza HR (2012) Determination of nitrite in meat products and water using dapsone with combined spot test/diffuse reflectance on filter paper. Food Chem 134(4):2546–2551

    Article  Google Scholar 

  • Ministry of Health of the People's Republic of China (2010) GB 5009.33-2010. The national food safety standards-The determination of nitrate and nitrite in food. Chinese standard press, Beijing

    Google Scholar 

  • Mirvish SS (1995) Role of N-nitroso compounds (NOC) and N-nitrosation in etiology of gastric, esophageal, nasopharyngeal and bladder cancer and contribution to cancer of known exposures to NOC. Cancer Lett 93(1):17–48

    Article  CAS  Google Scholar 

  • Pereira EA, Petruci JFS, Cardoso AA (2012) Determination of nitrite and nitrate in brazilian meats using high shear homogenization. Food Anal Method 5(4):637–642

    Article  Google Scholar 

  • Pourreza N, Fat'hi MR, Hatami A (2012) Indirect cloud point extraction and spectrophotometric determination of nitrite in water and meat products. Microchem J 104:22–25

    Article  CAS  Google Scholar 

  • Sabatte G, Keir R, Lawlor M, Black M, Graham D, Smith WE (2008) Comparison of surface-enhanced resonance Raman scattering and fluorescence for detection of a labeled antibody. Anal Chem 80(7):2351–2356

    Article  CAS  Google Scholar 

  • Socrates G, Socrates G (2001) Infrared and Raman characteristic group frequencies: tables and charts. Wiley, Chichester

    Google Scholar 

  • Thomas D, Rajith L, Lonappan L, Issac S, Kumar KG (2012) Sensitive determination of nitrite in food samples using voltammetric techniques. Food Anal Method 5(4):752–758

    Article  Google Scholar 

  • Unnikrishnan B, Ru P-L, Chen S-M, Mani V (2013) Nitrite determination at electrochemically synthesized polydiphenylamine-Pt composite modified glassy carbon electrode. Sensor Actuat B 177:887–892

    Article  CAS  Google Scholar 

  • U.S. Environmental Protection Agency (2009) Drinking water contaminants at http://water.epa.gov/drink/contaminants/index.cfm

  • Wang N, Wang RQ, Zhu Y (2012a) A novel ion chromatography cycling-column-switching system for the determination of low-level chlorate and nitrite in high salt matrices. J Hazard Mater 235:123–127

    Article  Google Scholar 

  • Wang P, Mai Z, Dai Z, Li Y, Zou X (2009) Construction of Au nanoparticles on choline chloride modified glassy carbon electrode for sensitive detection of nitrite. Biosens Bioelectron 24(11):3242–3247

    Article  CAS  Google Scholar 

  • Wang X, Adams E, Van Schepdael A (2012b) A fast and sensitive method for the determination of nitrite in human plasma by capillary electrophoresis with fluorescence detection. Talanta 97:142–144

    Article  CAS  Google Scholar 

  • Xi K, Sharma SK, Taylor GT, Muenow DW (1992) Determination of low concentrations of the azo-dye complex of nitrite in freshwater and Seawater using surface-enhanced resonance Raman spectroscopy (SERRS). Appl Spectrosc 46(5):819–826

    Article  CAS  Google Scholar 

  • Xian H, Wang P, Zhou Y, Lu Q, Wu S, Li Y, Wang L (2010) Electrochemical determination of nitrite via covalent immobilization of a single-walled carbon nanotubes and single stranded deoxyribonucleic acid nanocomposite on a glassy carbon electrode. Microchim Acta 171(1–2):63–69

    Article  CAS  Google Scholar 

  • Xie Y, Mukamurezi G, Sun Y, Wang H, Qian H, Yao W (2012) Establishment of rapid detection method of methamidophos in vegetables by surface enhanced Raman spectroscopy. Eur Food Res Technol 234(6):1091–1098

    Article  CAS  Google Scholar 

  • Zhang H, Zhang L, Lu C, Zhao L, Zheng Z (2012a) CdTe nanocrystals-enhanced chemiluminescence from peroxynitrous acid-carbonate and its application to the direct determination of nitrite. Spectrochim Acta A 85(1):217–222

    Article  CAS  Google Scholar 

  • Zhang X, Zou M, Qi X, Liu F, Zhu X, Zhao B (2010) Detection of melamine in liquid milk using surface-enhanced Raman scattering spectroscopy. J Raman Spectrosc 41(12):1655–1660

    Article  Google Scholar 

  • Zhang Y, Lai K, Zhou J, Wang X, Rasco BA, Huang Y (2012b) A novel approach to determine leucomalachite green and malachite green in fish fillets with surface-enhanced Raman spectroscopy (SERS) and multivariate analyses. J Raman Spectrosc 43(9):1208–1213

    CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by the National “Twelfth Five-Year” Plan for Science and Technology Support (No. 2012BAF14B08), Program for New Century Excellent Talents in University (No. NECT-10-0443), National Natural Science Foundation of China (Nos. 21075049 and 21105037), and Science and Technology Developing Foundation of Jilin Province (Nos. 20121808 and 20125006).

Conflict of Interest

Pinyi Ma declares that he has no conflict of interest. Fanghui Liang declares that she has no conflict of interest. Xinpei Li declares that she has no conflict of interest. Qingqing Yang declares that she has no conflict of interest. Di Wang declares that she has no conflict of interest. Daqian Song declares that he has no conflict of interest. Dejiang Gao declares that he has no conflict of interest. Xinghua Wang declares that he has no conflict of interest. This article does not contain any studies with human or animal subjects.

Author information

Authors and Affiliations

  1. College of Chemistry, Jilin University, Qianjin Street 2699, Changchun, 130012, People’s Republic of China

    Pinyi Ma, Xinpei Li, Qingqing Yang, Di Wang, Daqian Song & Xinghua Wang

  2. Department of Pharmacy, Changchun Medical College, Jilin Street 6177, Changchun, 130031, People’s Republic of China

    Fanghui Liang

  3. Changchun Jilin University Little Swan Instruments Co., Ltd., Chuangxin Road 1203, Changchun, 130012, People’s Republic of China

    Dejiang Gao

Authors
  1. Pinyi Ma
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Fanghui Liang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Xinpei Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Qingqing Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Di Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Daqian Song
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Dejiang Gao
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Xinghua Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Xinghua Wang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ma, P., Liang, F., Li, X. et al. Development and Optimization of a SERS Method for On-site Determination of Nitrite in Foods and Water. Food Anal. Methods 7, 1866–1873 (2014). https://doi.org/10.1007/s12161-014-9829-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12161-014-9829-3

Keywords

Search

Navigation