Abstract
A rapid method for detection of flumequine (FQ) and oxolinic acid (OA) residues from fish tissues was established using pressurized capillary electrochromatography (pCEC). Residual FQ and OA were extracted from fish tissue samples with acidified acetonitrile and purified on an Oasis HLB C18 solid-phase extraction column. The extract was then analyzed by pCEC with the mobile phase (pH = 3) consisting of acetonitrile and 15 mM ammonium acetate (45: 55, v/v, respectively) at the flow rate of 0.07 mL/min, detection wavelength of 324 nm, and applied voltage of −10 kV. Baseline separation of FQ and OA was achieved in 10 min; both drugs showed good linearity in the range of 0.2–5 μg/mL. The recoveries of FQ and OA were between 82 and 92 % (relative standard deviation = 3.63–5.96). The limits of detection for FQ and OA were 0.1 and 0.08 mg/kg, respectively.
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Acknowledgments
This work was financially supported by the National Natural Science Foundation of China (21276240) and Zhejiang Provincial Natural Science Foundation of China (Z3110487 and Y2111223); Funds of Zhoushan Science and technology bureau (2011C22044, 2013C41006).
Compliance with Ethics Requirements
The authors declare that the design, performance, and reporting of research funded under the National Natural Science Foundation of China (21276240) and Zhejiang Provincial Natural Science Foundation of China (Z3110487 and Y2111223); Funds of Zhoushan Science and technology bureau (2011C22044, 2013C41006) are free from bias resulting from investigator financial conflicts of interest. No financial relationship with other institutions or private industry has influenced the results of this study. All institutional and national guidelines for the care and use of laboratory animals were followed.
Conflict of Interest
Xiao-kun OuYang declares that he has no conflict of interest. Yu-Yang Luo declares that she has no conflict of interest. Zheng-Shun Wen declares that he has no conflict of interest. Wei-Jian Wu declares that he has no conflict of interest. Guo-Zhou Cao declares that he has no conflict of interest. Xiao-Yan Zhu declares that she has no conflict of interest. Li-ye Yang declares that he has no conflict of interest. Yang-Guang Wang declares that he has no conflict of interest. Jie-Ying Dong declares that she has no conflict of interest.
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OuYang, Xk., Luo, YY., Wen, ZS. et al. Simultaneous Determination of Flumequine and Oxolinic Acid Residues in Aquatic Products Using Pressurized Capillary Electrochromatography. Food Anal. Methods 7, 1770–1775 (2014). https://doi.org/10.1007/s12161-014-9818-6
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DOI: https://doi.org/10.1007/s12161-014-9818-6